Application | Comment | Organism |
---|---|---|
biotechnology | study presents a simple and cost-effective procedure for a large-scale production | Bos taurus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as glutathione S-transferase-fusion protein, the gene is a Pro82Arg/Glu176Asp variant of the known bovine EK | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
SDS-PAGE, MALDI-TOF | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q6B4R4 | chinese northern yellow bovine | - |
Purification (Comment) | Organism |
---|---|
of the recombinant fusion protein using nickel affinity column chromatography, refolding and auto-catalytic cleavage | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
duodenum | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione S-transferase-enterokinase + H2O | GST-EK harboring an EK site | Bos taurus | enterokinase + glutathione S-transferase | - |
? | |
GST-GFPuv + H2O | harboring an EK site between GST and the green fluorescent protein GFPuv | Bos taurus | GST + GFPuv | - |
? | |
trypsinogen + H2O | - |
Bos taurus | trypsin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enterokinase | EK | Bos taurus |