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Literature summary for 3.4.21.9 extracted from

  • Kubitzki, T.; Noll, T.; Luetz, S.
    Immobilisation of bovine enterokinase and application of the immobilised enzyme in fusion protein cleavage (2008), Bioprocess Biosyst. Eng., 31, 173-182.
    View publication on PubMed
Search for more literature for 3.4.21.9

Application

Application Comment Organism
biotechnology EK is immobilised on hexamethylamino Sepabeads or on amino-modified paramagnetic microspheres. 50% of activity remains after immobilisation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Storage Stability

Storage Stability Organism
The free enzyme has a half life of 8 days at 23°C. The immobilised enzyme has a half life of 29 days at 23°C. The immobilised and glutardialdehyde treated enzyme has a half life of 36 days at 23°C. The free enzyme has a half life of 85 days at 4°C. The immobilised enzyme has a half life of 10 month at 4°C. Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
 Bos taurus Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?
MUC1-IgG Fc + H2O fusion protein Bos taurus MUC1 + IgG Fc
-
 ?

Synonyms

Synonyms Comment Organism
enteropeptidase EK Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 immobilised enzyme on hexamethylamino Sepabeads or paramagnetic microspheres Bos taurus
33
-
 free enzyme Bos taurus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 40
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 immobilised enzyme on paramagnetic microspheres Bos taurus
8
-
 free enzyme Bos taurus
9
-
 immobilised enzyme on hexamethylamino Sepabeads Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Bos taurus