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Literature summary for 3.4.21.9 extracted from

  • Peng, L.; Zhong, X.; Ou, J.; Zheng, S.; Liao, J.; Wang, L.; Xu, A.
    High-level secretory production of recombinant bovine enterokinase light chain by Pichia pastoris (2004), J. Biotechnol., 108, 185-192.
    View publication on PubMed
Search for more literature for 3.4.21.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris strain GS115 mutants Mut+ and MutS Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 SDS-PAGE, recombinant enterokinase light chain Bos taurus
44000
-
 SDS-PAGE, His-tagged recombinant enterokinase light chain Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange chromatography Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8000
-
 rEKL/His, Gly-Asp-Asp-Asp-Asp-Lys-beta-naphthylamide substrate Bos taurus
9000
-
 rEKL, Gly-Asp-Asp-Asp-Asp-Lys-beta-naphthylamide substrate Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Asp-Asp-Asp-Asp-Lys-2-naphthylamide + H2O
-
 Bos taurus Gly-Asp-Asp-Asp-Asp-Lys + 2-naphthylamine
-
 ?

Synonyms

Synonyms Comment Organism
EK
-
 Bos taurus
enterokinase
-
 Bos taurus
recombinant enterokinase light chain
-
 Bos taurus
recombinant His-tagged enterokinase light chain
-
 Bos taurus
rEKL
-
 Bos taurus
rEKL/His
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5
-
 Bos taurus