Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
R222A | kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value. Thermostability is significantly lower compared to the wild-type enzyme | Escherichia coli |
R222K | kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value. Thermostability is significantly lower compared to the wild-type enzyme | Escherichia coli |
R315A | kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value. Thermostability is significantly lower compared to the wild-type enzyme | Escherichia coli |
R318A | kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value | Escherichia coli |
R318K | kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value. Thermostability is significantly lower compared to the wild-type enzyme | Escherichia coli |
R77A | kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value | Escherichia coli |
R77K | kcat is about 5.5fold smaller than the wild-type value, Km-is almost identical to wild-type value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R318A | Escherichia coli | |
0.49 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R127A | Escherichia coli | |
0.49 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R275A | Escherichia coli | |
0.49 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R282A | Escherichia coli | |
0.49 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R315K | Escherichia coli | |
0.52 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R146A | Escherichia coli | |
0.52 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R198A | Escherichia coli | |
0.52 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R226A | Escherichia coli | |
0.52 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R236A | Escherichia coli | |
0.52 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R295A | Escherichia coli | |
0.52 | - |
FSASALAKI | 37°C, pH 7.7, wild-type enzyme | Escherichia coli | |
0.53 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R222K | Escherichia coli | |
0.53 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R318A | Escherichia coli | |
0.54 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R222A | Escherichia coli | |
0.55 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R199A | Escherichia coli | |
0.57 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R315A | Escherichia coli | |
0.63 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R77K | Escherichia coli | |
0.67 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R77A | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
x * 37000, wild-type and mutant enzymes, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FSASALAKI + H2O | - |
Escherichia coli | FSASALA + Lys-Ile | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37000, wild-type and mutant enzymes, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R318A | Escherichia coli | |
0.08 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R318A | Escherichia coli | |
0.248 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R77A | Escherichia coli | |
0.27 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R222A | Escherichia coli | |
0.32 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R222K | Escherichia coli | |
0.42 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R315A | Escherichia coli | |
0.71 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R77K | Escherichia coli | |
0.96 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R226A | Escherichia coli | |
1 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R275A | Escherichia coli | |
1.02 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R315K | Escherichia coli | |
1.11 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R127A | Escherichia coli | |
1.11 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R198A | Escherichia coli | |
1.2 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R282A | Escherichia coli | |
1.2 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R295A | Escherichia coli | |
1.31 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R199A | Escherichia coli | |
1.31 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R236A | Escherichia coli | |
1.44 | - |
FSASALAKI | 37°C, pH 7.7, mutant enzyme R146A | Escherichia coli | |
1.44 | - |
FSASALAKI | 37°C, pH 7.7, wild-type enzyme | Escherichia coli |