Any feedback?
Literature summary for 3.4.21.85 extracted from
- Purification and properties of intracellular clotting factor, factor B, from horseshoe crab (Tachypleus tridentatus) hemocytes (1986), J. Biochem., 99, 847-857.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | factor B is activated by Limulus clotting factor C | Tachypleus tridentatus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha2-Plasmin inhibitor | - |
Tachypleus tridentatus | |
| benzamidine | - |
Tachypleus tridentatus |  |
| DFP | - |
Tachypleus tridentatus | |
| leupeptin | - |
Tachypleus tridentatus | |
| PCMB | - |
Tachypleus tridentatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol | Tachypleus tridentatus |
| 25000 | - |
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions | Tachypleus tridentatus |
| 32000 | - |
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions | Tachypleus tridentatus |
| 40000 | - |
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol | Tachypleus tridentatus |
| 56000 | - |
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions | Tachypleus tridentatus |
| 64000 | - |
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol | Tachypleus tridentatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Tachypleus tridentatus | 3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tachypleus tridentatus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tachypleus tridentatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hemocyte | - |
Tachypleus tridentatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Benzoyl-Ser-Ser-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Benzoyl-Ser-Thr-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Benzoyl-Thr-Ser-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Benzoyl-Thr-Thr-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Limulus proclotting enzyme + H2O | - |
Tachypleus tridentatus | Active clotting enzyme | - |
? | |
| additional information | 3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme | Tachypleus tridentatus | ? | - |
? | |
| N-tert-Butoxycarbonyl-Met-Thr-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Tert-Butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
| Tert-Butoxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions | Tachypleus tridentatus |
| More | factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol | Tachypleus tridentatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 24 h, stable, factor B zymogen | Tachypleus tridentatus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
