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show all sequences of 3.4.21.85

Purification and properties of intracellular clotting factor, factor B, from horseshoe crab (Tachypleus tridentatus) hemocytes

Nakamura, T.; Horiuchi, T.; Morita, T.; Iwanaga, S.; J. Biochem. 99, 847-857 (1986)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
factor B is activated by Limulus clotting factor C
Tachypleus tridentatus
Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha2-Plasmin inhibitor
-
Tachypleus tridentatus
benzamidine
-
Tachypleus tridentatus
DFP
-
Tachypleus tridentatus
leupeptin
-
Tachypleus tridentatus
PCMB
-
Tachypleus tridentatus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions; factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
Tachypleus tridentatus
32000
-
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions
Tachypleus tridentatus
40000
-
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
Tachypleus tridentatus
56000
-
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions
Tachypleus tridentatus
64000
-
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
Tachypleus tridentatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Tachypleus tridentatus
3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Tachypleus tridentatus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Tachypleus tridentatus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
hemocyte
-
Tachypleus tridentatus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Benzoyl-Ser-Ser-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Benzoyl-Ser-Thr-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Benzoyl-Thr-Ser-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Benzoyl-Thr-Thr-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Limulus proclotting enzyme + H2O
-
29793
Tachypleus tridentatus
Active clotting enzyme
-
-
-
-
additional information
3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme
29793
Tachypleus tridentatus
?
-
-
-
-
N-tert-Butoxycarbonyl-Met-Thr-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Tert-Butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Tert-Butoxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions
Tachypleus tridentatus
More
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
Tachypleus tridentatus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
9
24 h, stable, factor B zymogen
Tachypleus tridentatus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
factor B is activated by Limulus clotting factor C
Tachypleus tridentatus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha2-Plasmin inhibitor
-
Tachypleus tridentatus
benzamidine
-
Tachypleus tridentatus
DFP
-
Tachypleus tridentatus
leupeptin
-
Tachypleus tridentatus
PCMB
-
Tachypleus tridentatus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions; factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
Tachypleus tridentatus
32000
-
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions
Tachypleus tridentatus
40000
-
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
Tachypleus tridentatus
56000
-
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions
Tachypleus tridentatus
64000
-
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
Tachypleus tridentatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Tachypleus tridentatus
3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Tachypleus tridentatus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
hemocyte
-
Tachypleus tridentatus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Benzoyl-Ser-Ser-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Benzoyl-Ser-Thr-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Benzoyl-Thr-Ser-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Benzoyl-Thr-Thr-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Limulus proclotting enzyme + H2O
-
29793
Tachypleus tridentatus
Active clotting enzyme
-
-
-
-
additional information
3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme
29793
Tachypleus tridentatus
?
-
-
-
-
N-tert-Butoxycarbonyl-Met-Thr-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Tert-Butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Tert-Butoxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
-
29793
Tachypleus tridentatus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions
Tachypleus tridentatus
More
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol
Tachypleus tridentatus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
9
24 h, stable, factor B zymogen
Tachypleus tridentatus
Other publictions for EC 3.4.21.85
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
665465
Muta
-
Horseshoe Crab Coagulation Fac ...
Tachypleus tridentatus
J. Biol. Chem.
268
2138-21388
1993
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1
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1
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1
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2
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-
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1
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1
-
-
-
-
-
-
2
-
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29793
Nakamura
Purification and properties of ...
Tachypleus tridentatus
J. Biochem.
99
847-857
1986
1
-
-
-
-
-
5
-
-
-
5
1
-
2
-
-
1
-
-
1
-
-
11
2
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
5
-
-
-
-
5
1
-
-
-
1
-
1
-
-
11
2
-
-
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-
-
-
1
-
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