Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | factor B is activated by Limulus clotting factor C | Tachypleus tridentatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha2-Plasmin inhibitor | - |
Tachypleus tridentatus | |
benzamidine | - |
Tachypleus tridentatus | |
DFP | - |
Tachypleus tridentatus | |
leupeptin | - |
Tachypleus tridentatus | |
PCMB | - |
Tachypleus tridentatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol | Tachypleus tridentatus |
25000 | - |
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions | Tachypleus tridentatus |
32000 | - |
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions | Tachypleus tridentatus |
40000 | - |
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol | Tachypleus tridentatus |
56000 | - |
1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions | Tachypleus tridentatus |
64000 | - |
factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol | Tachypleus tridentatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Tachypleus tridentatus | 3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tachypleus tridentatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Tachypleus tridentatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hemocyte | - |
Tachypleus tridentatus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzoyl-Ser-Ser-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Benzoyl-Ser-Thr-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Benzoyl-Thr-Ser-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Benzoyl-Thr-Thr-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Limulus proclotting enzyme + H2O | - |
Tachypleus tridentatus | Active clotting enzyme | - |
? | |
additional information | 3 protease zymogens, factor C, factor B, and proclotting enzyme are required for the sequential activation of the limulus clotting system. Factor B is activated by factor C (sensitive to lipopolysaccharide) and the resulting factor B activates proclotting enzyme to the clotting enzyme | Tachypleus tridentatus | ? | - |
? | |
N-tert-Butoxycarbonyl-Met-Thr-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
tert-butoxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Tert-Butoxycarbonyl-Leu-Thr-Arg 4-methylcoumarin 7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? | |
Tert-Butoxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O | - |
Tachypleus tridentatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 32000 + 1 * 25000, Tachypleus tridentatus, SDS-PAGE under reducing conditions, 1 * 56000, Tachypleus tridentatus, SDS-PAGE under nonreducing conditions | Tachypleus tridentatus |
More | factor B (zymogen) is a mixture of single-chain and two-chain forms: x * 64000, Tachypleus tridentatus, SDS-PAGE without 2-mercaptoethanol, x * 64000 + x * 40000 + x * 25000, Tachypleus tridentatus, SDS-PAGE after reduction with 2-mercaptoethanol | Tachypleus tridentatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | 24 h, stable, factor B zymogen | Tachypleus tridentatus |