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Literature summary for 3.4.21.84 extracted from

  • Nakamura, T.; Morita, T.; Iwanaga, S.
    Lipopolysaccharide-sensitive serine-protease zymogen (factor C) found in Limulus hemocytes. Isolation and characterization (1986), Eur. J. Biochem., 154, 511-521.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
additional information the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide Limulus polyphemus
additional information the zymogen factor C shows autocatalytic activation in presence of bacterial lipopolysaccharide Tachypleus sp.

Inhibitors

Inhibitors Comment Organism Structure
antithrombin III
-
 Tachypleus sp.
benzamidine
-
 Tachypleus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol Limulus polyphemus
43000
-
 x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol Tachypleus sp.
80000
-
 x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol Limulus polyphemus
80000
-
 x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol Tachypleus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Limulus polyphemus
-
 ?
-
 ?
additional information Tachypleus sp. factor C is essential for the endotoxin-mediated coagulation system ?
-
 ?

Organism

Organism UniProt Comment Textmining
Limulus polyphemus
-
-
-
Tachypleus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information one enzyme molecule contains 24 residues of hexose and 31 residues of hexosamine Tachypleus sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Tachypleus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Tachypleus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
 Limulus polyphemus ?
-
 ?
additional information factor C is essential for the endotoxin-mediated coagulation system Tachypleus sp. ?
-
 ?
N-tert-Butoxycarbonyl-Leu-Gly-Arg 4-nitroanilide + H2O
-
 Tachypleus sp. ?
-
 ?
N-tert-Butoxycarbonyl-Thr-Ser-Arg 4-nitroanilide + H2O
-
 Tachypleus sp. ?
-
 ?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O
-
 Limulus polyphemus ?
-
 ?
N-tert-Butoxycarbonyl-Val-Pro-Arg 4-nitroanilide + H2O
-
 Tachypleus sp. ?
-
 ?
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
 Limulus polyphemus ?
-
 ?
tert-butoxycarbonyl-Asp-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
 Tachypleus sp. ?
-
 ?
tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O
-
 Limulus polyphemus ?
-
 ?
tert-butoxycarbonyl-Glu(O-benzyl)-Gly-Arg 4-methylcoumarin 7-amide + H2O
-
 Tachypleus sp. ?
-
 ?
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
 Limulus polyphemus ?
-
 ?
Tert-Butoxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O
-
 Tachypleus sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol Limulus polyphemus
? x * 80000 + x * 43000, Limulus polyphemus, SDS-PAGE after reduction with 2-mercaptoethanol Tachypleus sp.
? upon activation cleavage of the light chain occurs resulting in the accumulation of two new fragments of MW 34000 and 8500 on reduced SDS-PAGE Limulus polyphemus
? upon activation cleavage of the light chain occurs resulting in the accumulation of two new fragments of MW 34000 and 8500 on reduced SDS-PAGE Tachypleus sp.