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Literature summary for 3.4.21.83 extracted from

  • Usuki, H.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
    Activation of oligopeptidase B from Streptomyces griseus by thiol-reacting reagents is independent of the single reactive cysteine residue (2009), Biochim. Biophys. Acta, 1794, 1673-1683.
    View publication on PubMed
Search for more literature for 3.4.21.83

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol enzymatic activity is markedly enhanced by dithiothreitol at submillimolar level, which affects the P1 preference of OpdB synergistically in a substrate-dependent manner Streptomyces griseus
NaCl enzymatic activity is markedly enhanced by high NaCl concentration, NaCl affects the P1 preference of OpdB synergistically in a substrate-dependent manner Streptomyces griseus
oxidized glutathione oxidized glutathione enhances OpdB activity and affects the P1 preference of OpdB synergistically in a substrate-dependent manner Streptomyces griseus
reduced glutathione enzymatic activity is markedly enhanced by reduced glutathione at submillimolar level which affects the P1 preference of OpdB synergistically in a substrate-dependent manner Streptomyces griseus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells Streptomyces griseus

Protein Variants

Protein Variants Comment Organism
C544A the enzymatic activity of the Cys-free mutant is markedly activated by the general thiol-reacting reagent dithiothreitol, oxidized glutathione, and reduced glutathione,the mutant is insensitive to the treatment of the SH-blocking reagent N-ethylmaleimide or H2O2 Streptomyces griseus

Inhibitors

Inhibitors Comment Organism Structure
4-(2-aminoethyl)benzenesulfonyl fluoride OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 4 mM pefbloc SC Streptomyces griseus
antipain OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 0.3 mM antipain Streptomyces griseus
leupeptin OpdB activity toward tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin hydrolysis is inhibited completely by 0.05 mM leupeptin Streptomyces griseus
additional information OpdB is not inhibited by E-64 (0.03 mM), pepstain (0.04 mM), EDTA (1 mM), chimostatin (0.2 mM), and phosphoramidon (0.4 mM) Streptomyces griseus
N-ethylmaleimide
-
 Streptomyces griseus
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin in the case of added dithiothreitol, reduced glutathione, or oxidized glutathione, a high concentration of the substrate tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin inhibits the enzyme activity. By contrast, addition of 1 M NaCl to either dithiothreitol, reduced glutathione, or oxidized glutathione or 1 M NaCl alone prevents substrate inhibition Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TALON metal affinity resin column chromatography Streptomyces griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Streptomyces griseus benzyloxycarbonyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Streptomyces griseus benzyloxycarbonyl-L-Leu-L-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Streptomyces griseus benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Streptomyces griseus benzyloxycarbonyl-L-Val-L-Val-L-Arg + 7-amino-4-methylcoumarin
-
 ?
CBZ-L-Arg-L-Arg-7-amido-4-methylcoumarin + H2O weak substrate Streptomyces griseus CBZ-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
L-pGlu-Gly-L-Arg-7-amido-4-methylcoumarin + H2O low hydrolytic rate Streptomyces griseus L-pGlu-Gly-L-Arg + 7-amino-4-methylcoumarin
-
 ?
L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg-7-amido-4-methylcoumarin + H2O low hydrolytic rate Streptomyces griseus L-pGlu-L-Arg-L-Thr-L-Lys-L-Arg + 7-amino-4-methylcoumarin
-
 ?
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Streptomyces griseus L-Pro-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information no hydrolytic activity against the aminopeptidase substrates (4-nitroanilide derivatives of amino acids) and FITC-casein, substrates containing Pro at Xaa positions in the FRETS library are not hydrolyzed Streptomyces griseus ?
-
 ?
tert-butoxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin + H2O best substrate, the hydrolytic activity is observed only in the presence of NaCl and the thiol-reacting reagents dithiothreitol, reduced glutathione, and oxidized glutathione at pH 8.5 (50 mM Tris-HCl) Streptomyces griseus tert-butoxycarbonyl-L-Val-L-Leu-L-Lys + 7-amino-4-methylcoumarin
-
 ?
tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Streptomyces griseus tosyl-Gly-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
 ?
tosyl-Gly-L-Pro-L-Lys-7-amido-4-methylcoumarin + H2O
-
 Streptomyces griseus tosyl-Gly-L-Pro-L-Lys + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
oligopeptidase B
-
 Streptomyces griseus
OpdB
-
 Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 pH optimum in the presence of NaCl Streptomyces griseus
8.8
-
 pH optimum in the absence of NaCl Streptomyces griseus