Cloned (Comment) | Organism |
---|---|
fusion expression of oligopeptidase B with an N-terminal histidine tag using pET28a as the expression vector. Although most of the recombinant OpdB is produced as inclusion bodies, the solubility of the recombinant protease increases significantly when expression temperature shifts from 37°C to 30°C | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester | IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbobenzoxy-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Escherichia coli | ? | - |
? | |
Nalpha-benzoyl-Arg-7-amido-4-methylcoumarin + H2O | 68% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide | Escherichia coli | ? | - |
? | |
tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin + H2O | specific substrate for Escherichia coli oligopeptidase B, 20% of the activity with carbobenzoxy-Arg-Arg-4-methylcoumaryl-7-amide | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OpdB | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
15 min, stable below | Escherichia coli |
50 | - |
15 min, complete inactivation | Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
IC50: about 0.01 mM. Oligopeptidase B is much more sensitive than oligopeptidase B | Escherichia coli | trans-4-guanidinomethylcyclohexanecarboxylic acid 4-tert-butylphenyl ester |