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Literature summary for 3.4.21.83 extracted from

  • Morty, R.E.; Fueloep, V.; Andrews, N.W.
    Substrate recognition properties of oligopeptidase B from Salmonella enterica serovar Typhimurium (2002), J. Bacteriol., 184, 3329-3337.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.83

Application

Application Comment Organism
medicine potential theraputic target in infectious disease Salmonella enterica

Cloned(Commentary)

Cloned (Comment) Organism
opdB gene PCR amplified and expressed in Escherichia coli BL21(lambdaDE3) Salmonella enterica

Protein Variants

Protein Variants Comment Organism
D460T site-mutated variant Salmonella enterica
D460T/D462N site-mutated variant Salmonella enterica
D462N site-mutated variant Salmonella enterica
D567H site-mutated variant Salmonella enterica
D599H site-mutated variant Salmonella enterica
D638Q site-mutated variant Salmonella enterica
E494H site-mutated variant Salmonella enterica
E576A site-mutated variant Salmonella enterica
E576A/E578A site-mutated variant Salmonella enterica
E576R/E578R site-mutated variant Salmonella enterica
E576W/E578T site-mutated variant Salmonella enterica
E578A site-mutated variant Salmonella enterica
E624H site-mutated variant Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin irreversible inhibition Salmonella enterica
4-(2-aminoethyl)benzenesulfonyl fluoride irreversible inhibition Salmonella enterica
antipain reversible inhibition Salmonella enterica
benzamidine reversible inhibition Salmonella enterica
leupeptin reversible inhibition Salmonella enterica
additional information no inhibition of wild-type enzyme with iodoacetamide, iodoacetate, N-ethylmaleimide, EDTA, EGTA, bestatin,amastatin, arphamenine A, elastinal, dynorphin A or dynorphin B Salmonella enterica
p-aminobenzamidine reversible inhibition Salmonella enterica
phenylmethane sulfonyl fluoride irreversible inhibition Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, wild-type and mutant D567H Salmonella enterica
0.00088
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, opdB with polyhistidine affinty tag removed Salmonella enterica
0.001
-
 Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.001
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E494H, E624H, and D599H Salmonella enterica
0.0011
-
 Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0011
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
0.0019
-
 Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0019
-
 Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.002
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D567H Salmonella enterica
0.0021
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T Salmonella enterica
0.0021
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, wild-type and mutant D599A Salmonella enterica
0.0021
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, wild-type and mutant D599H Salmonella enterica
0.0022
-
 Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0023
-
 benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0023
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
0.0024
-
 Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0025
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E494H Salmonella enterica
0.0026
-
 benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0026
-
 Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0026
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E624H Salmonella enterica
0.0028
-
 Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0028
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
0.0029
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T and D460T/D462N Salmonella enterica
0.0029
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
0.003
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
0.0034
-
 benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.004
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
0.0044
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0045
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D599H Salmonella enterica
0.0045
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica
0.0046
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T and E494H Salmonella enterica
0.0047
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
0.005
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D567H Salmonella enterica
0.0051
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, opdB with polyhistidine affinty tag removed Salmonella enterica
0.0052
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
0.0054
-
 benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0054
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E624H Salmonella enterica
0.0059
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E578A Salmonella enterica
0.0081
-
 Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.0099
-
 Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
0.012
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica
0.0164
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E578A Salmonella enterica
0.0334
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78970
-
 predicted from opdB gene open reading frame Salmonella enterica

Organism

Organism UniProt Comment Textmining
Salmonella enterica A0A0H3NCA6 serovar typhimurium, virulent wild-type strain SL1344
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica Boc-Gly-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica Boc-Gly-Lys-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
Boc-Leu-Lys-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
histone + H2O human histones H1, H2A, H3 and H4, restricted proteolysis Salmonella enterica ?
-
 ?
additional information exhibits amidolytic activity exclusively against substrates with basic residues in P1, no activity is observed against H-Arg-4-methylcoumarin 7-amide, H-Leu-4-methylcoumarin 7-amide, H-Lys-4-methylcoumarin 7-amide, benzyloxycarbonyl-Leu-Leu-Glu-beta-nitroanilide, benzyloxycarbonyl-Gly-Gly-Leu-p-nitroanilide, benzyloxycarbonyl-Ala-Ala-Phe-4-methylcoumarin 7-amide, Suc-Ile-Ala-4-methylcoumarin 7-amide, Suc-Gly-Pro-4-methylcoumarin 7-amide, Ac-Tyr-Val-Ala-Asp-p-nitroanilide, Suc-Ala-Ala-Pro-Phe-4-methylcoumarin 7-amide Salmonella enterica ?
-
 ?
Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?
Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide + H2O
-
 Salmonella enterica ?
-
 ?

Synonyms

Synonyms Comment Organism
OpdB
-
 Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
120
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica
240
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E578A Salmonella enterica
420
-
 benzyloxycarbonyl-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
420
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
420
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A Salmonella enterica
540
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E576A and E578A Salmonella enterica
540
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E578A Salmonella enterica
540
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, wild-type, mutant E494H, D599H and D567H Salmonella enterica
600
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, opdB with polyhistidine affinity tag removed, mutant D460T, D462N and E624H Salmonella enterica
660
-
 benzyloxycarbonyl-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
780
-
 Tos-Gly-Pro-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
900
-
 benzyloxycarbonyl-Val-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
900
-
 benzyloxycarbonyl-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
960
-
 Boc-Leu-Gly-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1020
-
 Boc-Gln-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1020
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
1080
-
 Boc-Leu-Thr-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1080
-
 Boc-Leu-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1080
-
 Tos-Gly-Pro-Lys-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1080
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T and E494H Salmonella enterica
1080
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T/D462N Salmonella enterica
1140
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
1200
-
 Boc-Gly-Lys-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1200
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D599A and D638Q Salmonella enterica
1260
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1320
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D460T Salmonella enterica
1320
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D567H and E624H Salmonella enterica
1380
-
 Boc-Gly-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1380
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D462N Salmonella enterica
1500
-
 benzyloxycarbonyl-Gly-Gly-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1560
-
 Boc-Leu-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C Salmonella enterica
1740
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C wild-type and mutant D567H Salmonella enterica
1800
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D599H Salmonella enterica
1860
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant D638Q Salmonella enterica
1980
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, mutant E494H and E624H Salmonella enterica
2040
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide pH 8.0, 37°C, opdB with polyhistidine affinty tag removed Salmonella enterica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000081
-
 antipain pH 8.0, 37°C Salmonella enterica
0.0004
-
 leupeptin pH 8.0, 37°C Salmonella enterica
0.1171
-
 p-aminobenzamidine pH 8.0, 37°C Salmonella enterica
0.1867
-
 benzamidine pH 8.0, 37°C Salmonella enterica