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Literature summary for 3.4.21.83 extracted from
- Protease II from Escherichia coli. Substrate specificity and kinetic properties (1978), Eur. J. Biochem., 82, 439-451.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DFP | - |
Escherichia coli |  |
| tosyl-Leu chloromethyl ketone | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
tosyl-Arg methyl ester | - |
Escherichia coli | |
| 0.31 | - |
N-Benzyloxycarbonyl-Lys benzyl ester | - |
Escherichia coli | |
| 0.33 | - |
N-Benzyloxycarbonyl-Lys benzyl ester | - |
Escherichia coli | |
| 0.47 | - |
tosyl-Lys-methyl ester | - |
Escherichia coli | |
| 0.5 | - |
benzoyl-Arg 4-nitroanilide | benzoyl-Arg ethyl ester | Escherichia coli | |
| 0.6 | - |
N-benzoyl-Arg amide | - |
Escherichia coli | |
| 80 | - |
acetyl-tyrosine 4-nitroanilide | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Acetyl-Tyr 4-nitroanilide + H2O | - |
Escherichia coli | Acetyl-Tyr + 4-nitroaniline | - |
? | |
| benzoyl-Arg-NH2 + H2O | - |
Escherichia coli | ? | - |
? | |
| Insulin B-chain + H2O | cleaves the carboxymethylated B-chain of insulin at the Arg22-Gly23 bond, but after prolonged periods of incubation it is also able to cleave the Tyr16-Leu17 bond | Escherichia coli | ? | - |
? | |
| additional information | not: luteinizing hormone releasing factor | Escherichia coli | ? | - |
? | |
| N-Benzoyl-DL-Arg 4-nitroanilide + H2O | - |
Escherichia coli | N-Benzoyl-DL-Arg + 4-nitroaniline | - |
? | |
| N-Benzoyl-L-Arg ethyl ester + H2O | - |
Escherichia coli | N-Benzoyl-L-Arg + ethanol | - |
? | |
| N-benzyloxycarbonyl-Lys benzyl ester + H2O | - |
Escherichia coli | N-benzyloxycarbonyl-Lys + benzyl alcohol | - |
? | |
| N-carbobenzoxy-L-Lys 4-nitrophenyl ester + H2O | - |
Escherichia coli | N-carbobenzoxy-L-Lys + 4-nitrophenol | - |
? | |
| Tosyl-Arg methyl ester + H2O | - |
Escherichia coli | Tosyl-Arg + methanol | - |
? | |
| Tosyl-Lys methyl ester + H2O | - |
Escherichia coli | Tosyl-Lys + benzyl alcohol | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
Acetyl-Tyr 4-nitroanilide | - |
Escherichia coli | |
| 3.5 | - |
N-benzoyl-arginine amide | - |
Escherichia coli | |
| 43 | - |
N-benzyloxycarbonyl-Lys 4-nitrophenyl ester | - |
Escherichia coli | |
| 55 | - |
Tosyl-Lys methyl ester | - |
Escherichia coli | |
| 55 | - |
N-Benzyloxycarbonyl-Lys benzyl ester | - |
Escherichia coli | |
| 57 | - |
N-benzoyl-arginine amide | - |
Escherichia coli | |
| 60 | - |
tosyl-Arg methyl ester | - |
Escherichia coli | |
| 197 | - |
N-benzoyl-Arg ethyl ester | - |
Escherichia coli | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
quick inactivation below | Escherichia coli |
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