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Literature summary for 3.4.21.81 extracted from

  • Qasim, M.A.; Wang, L.; Qasim, S.; Lu, S.; Lu, W.; Wynn, R.; Yi, Z.P.; Laskowski, M.
    Additivity-based design of the strongest possible turkey ovomucoid third domain inhibitors for porcine pancreatic elastase (PPE) and Streptomyces griseus protease B (SGPB) (2013), FEBS Lett., 587, 3021-3026.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
additional information design of inhibitors based on turkey ovomucoid third domain OMTKY3. Variant 13D14Y15G18I19K21-OMTKY3 displays a Gly residue in position P1 and represents the strongest possible inhibitor due to consensus contacts to the enzyme. Inhibitor has significantly higher DeltaGo value for protease B than that for protease A and inhibits protease B 10 times more strongly than protease A; OMTKY3 inhibitor variant screening, overview Streptomyces griseus
protein inhibitor S13D14Y15-OMTKY3 a OMTKY3 mutant variant, recombinantly produced in Escherichia coli strain RV308 and purified, strong inhibition, overview Streptomyces griseus
protein inhibitor S13D14Y15G18I19K21-OMTKY3 a OMTKY3 mutant variant, recombinantly produced in Escherichia coli strain RV308 and purified, strong inhibition, overview. S13D14Y15G18I19K21 OMTKY3 inhibits Streptomyces griseus protease B 10times more strongly than Streptomyces griseus protease A, EC 3.4.21.80 Streptomyces griseus

Organism

Organism UniProt Comment Textmining
Streptomyces griseus
-
-
-
Streptomyces griseus P00777
-
-

Purification (Commentary)

Purification (Comment) Organism
further purification of a commercial preparation Streptomyces griseus

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation requires further purification Streptomyces griseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
succinyl-Ala-Ala-Pro-X-4-nitroanilide + H2O
-
Streptomyces griseus ?
-
?
succinyl-Ala-Ala-Pro-X-7-amido-4-methylcoumarin + H2O
-
Streptomyces griseus ?
-
?

Synonyms

Synonyms Comment Organism
SGPB
-
Streptomyces griseus
SprB
-
Streptomyces griseus
Streptomyces griseus protease B
-
Streptomyces griseus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 8.3 assay at Streptomyces griseus