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Literature summary for 3.4.21.81 extracted from
- Active-site variants of Streptomyces griseus protease B with peptide-ligation activity (2000), Chem. Biol., 7, 163-171.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| active-site variant genes clones in the Escherichia coli/Bacillus subtilis shuttle vector pEB-11 and expressed in Bacillus subtilis DB104 | Streptomyces griseus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S195A | active-site variant of protease B with peptide-ligation activity | Streptomyces griseus |
| S195C | active-site variant of protease B with peptide-ligation activity | Streptomyces griseus |
| S195G | active-site variant of protease B with peptide-ligation activity | Streptomyces griseus |
| S195G/H57N | active-site variant of protease B with peptide-ligation activity | Streptomyces griseus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.062 | - |
Suc-AAPF-p-nitroanilide | pH 8.0, 25°C, native SGPB | Streptomyces griseus |  |
| 0.3 | - |
Suc-AAPF-p-nitroanilide | pH 8.0, 25°C, mutant S195G | Streptomyces griseus | |
| 0.5 | - |
Suc-AAPF-p-nitroanilide | pH 8.0, 25°C, mutant S195A | Streptomyces griseus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18600 | - |
- |
Streptomyces griseus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | 8 M, extremely stable | Streptomyces griseus |
| urea | 6 M, extremely stable | Streptomyces griseus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutants S195A and S195G | Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | member of the chymotrypsin superfamily of serine proteases | Streptomyces griseus | ? | - |
? | |
| Suc-AAPF-p-nitroanilide + H2O | - |
Streptomyces griseus | ? | - |
? | |
| Suc-AAPF-SBzl + H2O | - |
Streptomyces griseus | Suc-AAPF + phenylmethanethiol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 18600, SDS-PAGE | Streptomyces griseus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| protease B | - |
Streptomyces griseus |
| SGPB | - |
Streptomyces griseus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 45 | mutant S195A, completely inactivated in less than 1 min at 45°C, less than 20 min at 37°C | Streptomyces griseus |
| 55 | - |
native SGPB, half-life 8.3 min | Streptomyces griseus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.066 | - |
Suc-AAPF-p-nitroanilide | pH 8.0, 25°C, mutant S195G | Streptomyces griseus | |
| 0.474 | - |
Suc-AAPF-p-nitroanilide | pH 8.0, 25°C, mutant S195A | Streptomyces griseus | |
| 1880 | - |
Suc-AAPF-p-nitroanilide | pH 8.0, 25°C, native SGPB | Streptomyces griseus | |
| 1884 | - |
Suc-AAPF-p-nitroanilide | pH 8.0, 25°C, native SGPB | Streptomyces griseus | |
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