Cloned (Comment) | Organism |
---|---|
granzyme A expressed in Escherichia coli BL21-DE3 as a proenzyme capable of in vitro activation by enterokinase | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dichloroisocoumarin | weak inhibition | Homo sapiens | |
benzamidine | - |
Homo sapiens | |
C6H4CH2SO2-Gly-Pro(4-amidinophenylglycine)P(OPh)2 | - |
Homo sapiens | |
guanidinobenzoate | - |
Homo sapiens | |
isocoumarin | - |
Homo sapiens | |
p-guanidino-C6H4-COOC6H4-p-CN | - |
Homo sapiens | |
PhNH-CONH-CiTEtOIC | isocoumarinderivate, best inhibitor | Homo sapiens | |
phosphonate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
Boc-Trp-Arg-SBzl | pH 7.5, 30°C | Homo sapiens | |
0.177 | - |
Z-Arg-SBzl | pH 7.5, 30°C | Homo sapiens | |
0.767 | - |
Z-Lys-SBzl | pH 7.5, 30°C | Homo sapiens | |
0.887 | - |
Boc-Ala-Ala-Arg-SBzl | pH 7.5, 30°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | most abundant protease in cytotoxic T lymphocytes, granzyme A pathway of cell-mediated cytotoxicity, produced as proenzyme, activated by the dipetidyl exopeptidase cathepsin C | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
human | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O | - |
Homo sapiens | benzyloxycarbonyl-Lys + phenylmethanethiol | - |
? | |
Boc-Ala-Ala-Arg-SBzl + H2O | thioester substrate, recombinant granzyme A | Homo sapiens | ? | - |
? | |
Boc-Trp-Arg-SBzl + H2O | thioester substrate, recombinant granzyme A | Homo sapiens | ? | - |
? | |
CBZ-Lys-SBzl + H2O | thioester substrate, recombinant granzyme A | Homo sapiens | ? | - |
? | |
additional information | most abundant protease in cytotoxic T lymphocytes, granzyme A pathway of cell-mediated cytotoxicity, produced as proenzyme, activated by the dipetidyl exopeptidase cathepsin C | Homo sapiens | ? | - |
? | |
N-alpha-benzyloxycarbonyl-Arg-SBzl + H2O | thioester substrate, recombinant granzyme A | Homo sapiens | ? | - |
? | |
PHAPII + H2O | HLA-associated protein, cleaved by the recombinant enzyme at nanomolar concentrations | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | only granzyme that forms a disulfide-linked homodimer | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3960 | - |
Boc-Trp-Arg-SBzl | pH 7.5, 30°C | Homo sapiens | |
4980 | - |
Boc-Ala-Ala-Arg-SBzl | pH 7.5, 30°C | Homo sapiens | |
22100 | - |
Z-Lys-SBzl | pH 7.5, 30°C | Homo sapiens | |
22140 | - |
Z-Lys-SBzl | pH 7.5, 30°C | Homo sapiens | |
30360 | - |
Z-Arg-SBzl | pH 7.5, 30°C | Homo sapiens | |
30400 | - |
Z-Arg-SBzl | pH 7.5, 30°C | Homo sapiens |