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Literature summary for 3.4.21.78 extracted from

  • Beresford, P.J.; Kam, C.M.; Powers, J.C.; Lieberman, J.
    Recombinant human granzyme A binds to putative HLA-associated proteins and cleaves one of them (1997), Proc. Natl. Acad. Sci. USA, 94, 9285-9290.
    View publication on PubMed
Search for more literature for 3.4.21.78

Cloned(Commentary)

Cloned (Comment) Organism
granzyme A expressed in Escherichia coli BL21-DE3 as a proenzyme capable of in vitro activation by enterokinase Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin weak inhibition Homo sapiens
benzamidine
-
 Homo sapiens
C6H4CH2SO2-Gly-Pro(4-amidinophenylglycine)P(OPh)2
-
 Homo sapiens
guanidinobenzoate
-
 Homo sapiens
isocoumarin
-
 Homo sapiens
p-guanidino-C6H4-COOC6H4-p-CN
-
 Homo sapiens
PhNH-CONH-CiTEtOIC isocoumarinderivate, best inhibitor Homo sapiens
phosphonate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 Boc-Trp-Arg-SBzl pH 7.5, 30°C Homo sapiens
0.177
-
 Z-Arg-SBzl pH 7.5, 30°C Homo sapiens
0.767
-
 Z-Lys-SBzl pH 7.5, 30°C Homo sapiens
0.887
-
 Boc-Ala-Ala-Arg-SBzl pH 7.5, 30°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens most abundant protease in cytotoxic T lymphocytes, granzyme A pathway of cell-mediated cytotoxicity, produced as proenzyme, activated by the dipetidyl exopeptidase cathepsin C ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 human
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Lys-thiobenzyl ester + H2O
-
 Homo sapiens benzyloxycarbonyl-Lys + phenylmethanethiol
-
 ?
Boc-Ala-Ala-Arg-SBzl + H2O thioester substrate, recombinant granzyme A Homo sapiens ?
-
 ?
Boc-Trp-Arg-SBzl + H2O thioester substrate, recombinant granzyme A Homo sapiens ?
-
 ?
CBZ-Lys-SBzl + H2O thioester substrate, recombinant granzyme A Homo sapiens ?
-
 ?
additional information most abundant protease in cytotoxic T lymphocytes, granzyme A pathway of cell-mediated cytotoxicity, produced as proenzyme, activated by the dipetidyl exopeptidase cathepsin C Homo sapiens ?
-
 ?
N-alpha-benzyloxycarbonyl-Arg-SBzl + H2O thioester substrate, recombinant granzyme A Homo sapiens ?
-
 ?
PHAPII + H2O HLA-associated protein, cleaved by the recombinant enzyme at nanomolar concentrations Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer only granzyme that forms a disulfide-linked homodimer Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3960
-
 Boc-Trp-Arg-SBzl pH 7.5, 30°C Homo sapiens
4980
-
 Boc-Ala-Ala-Arg-SBzl pH 7.5, 30°C Homo sapiens
22100
-
 Z-Lys-SBzl pH 7.5, 30°C Homo sapiens
22140
-
 Z-Lys-SBzl pH 7.5, 30°C Homo sapiens
30360
-
 Z-Arg-SBzl pH 7.5, 30°C Homo sapiens
30400
-
 Z-Arg-SBzl pH 7.5, 30°C Homo sapiens