BRENDA - Enzyme Database show
show all sequences of 3.4.21.78

The oligomeric structure of human granzyme A is a determinant of its extended substrate specificity

Bell, J.K.; Goetz, D.H.; Mahrus, S.; Harris, J.L.; Fletterick, R.J.; Craik, C.S.; Nat. Struct. Biol. 10, 527-534 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cDNA subcloned into the yeast vector pPICzalphaA and heterologously expressed in Pichia pastoris
Homo sapiens
Crystallization (Commentary)
Crystallization
Organism
-
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
N153Q
molecular replacement
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
ecotin has no effect
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
cell death-inducing serine protease
?
-
Homo sapiens
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
human
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
cell death-inducing serine protease
653301
Homo sapiens
?
-
653301
Homo sapiens
?
PSSCL + H2O
-
653301
Homo sapiens
Pro-Ser + Ser-Lys-Lys
-
653301
Homo sapiens
?
Subunits
Subunits
Commentary
Organism
dimer
only granzyme that forms a disulfide-linked homodimer
Homo sapiens
oligomer
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
cDNA subcloned into the yeast vector pPICzalphaA and heterologously expressed in Pichia pastoris
Homo sapiens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
N153Q
molecular replacement
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
ecotin has no effect
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Homo sapiens
cell death-inducing serine protease
?
-
Homo sapiens
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
cell death-inducing serine protease
653301
Homo sapiens
?
-
653301
Homo sapiens
?
PSSCL + H2O
-
653301
Homo sapiens
Pro-Ser + Ser-Lys-Lys
-
653301
Homo sapiens
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
only granzyme that forms a disulfide-linked homodimer
Homo sapiens
oligomer
-
Homo sapiens
Other publictions for EC 3.4.21.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731617
Arias
Elucidating sources and roles ...
Mus musculus, Mus musculus B6
Cell Rep.
8
420-429
2014
-
1
-
-
-
-
-
-
-
-
-
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6
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3
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732177
Kaiserman
Identification of Serpinb6b as ...
Homo sapiens, Mus musculus
J. Biol. Chem.
289
9408-9417
2014
-
-
2
-
-
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3
-
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4
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2
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4
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2
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2
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
-
-
-
717504
Ilzecka
Granzymes A and B levels in se ...
Homo sapiens
Clin. Biochem.
44
650-653
2011
-
1
-
-
-
-
-
-
-
-
-
-
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2
-
-
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1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717675
Grodzovski
IL-2-granzyme A chimeric prote ...
Homo sapiens
Int. J. Cancer
128
1966-1980
2011
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717939
Hartmann
A novel and divergent role of ...
Mus musculus
J. Immunol.
186
2472-2481
2011
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
708274
Yoshikawa
Granzyme A and thrombin differ ...
Homo sapiens
Cytotechnology
62
325-332
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
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-
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-
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-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
717310
Van Damme
The substrate specificity prof ...
Homo sapiens
Biol. Chem.
391
983-997
2010
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
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1
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-
-
1
-
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-
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-
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-
-
-
-
-
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-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717616
Huang
Identification and characteriz ...
Cyprinus carpio
Fish Shellfish Immunol.
29
388-398
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
697233
Martinvalet
Granzyme A cleaves a mitochond ...
Homo sapiens
Cell
133
681-692
2008
1
-
1
-
1
-
3
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
3
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683058
Vernooy
Increased granzyme A expressio ...
Homo sapiens
Am. J. Respir. Crit. Care Med.
175
464-472
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683270
Hirayasu
A role of a lymphocyte tryptas ...
Rattus norvegicus
Biosci. Biotechnol. Biochem.
71
234-237
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
3
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683326
Baschuk
Interleukin-4 impairs granzyme ...
Mus musculus
Cancer Immunol. Immunother.
56
1625-1636
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683529
Hochegger
Expression of granzyme A in hu ...
Homo sapiens
Immunology
121
166-173
2007
1
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683723
Myoumoto
Glucocorticoid-induced granzym ...
Homo sapiens
J. Hum. Genet.
52
328-333
2007
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683789
Lee-Kirsch
A mutation in TREX1 that impai ...
Homo sapiens
J. Mol. Med.
85
531-537
2007
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664758
Zhu
Granzyme A, which causes singl ...
Homo sapiens
EMBO Rep.
7
431-437
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
-
-
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-
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1
-
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-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683201
Buzza
Extracellular granzymes: curre ...
Homo sapiens
Biol. Chem.
387
827-837
2006
-
1
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
683694
Kaiserman
The major human and mouse gran ...
Homo sapiens, Mus musculus
J. Cell Biol.
175
619-630
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
3
2
-
3
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
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-
-
-
-
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-
-
3
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664575
Mahrus
Selective chemical functional ...
Homo sapiens
Chem. Biol.
12
567-577
2005
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
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-
-
-
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2
-
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1
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1
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665142
Martinvalet
Granzyme A induces caspase-ind ...
Homo sapiens
Immunity
22
355-370
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
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1
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1
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1
-
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-
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-
-
-
665205
Bade
Differential expression of the ...
Homo sapiens
Int. Immunol.
17
1419-1428
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664445
Grossman
Differential expression of gra ...
Homo sapiens
Blood
104
2840-2848
2004
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665795
Pardo
Apoptotic pathways are selecti ...
Mus musculus
J. Cell Biol.
167
457-468
2004
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
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-
-
-
1
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1
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
665914
Shacklett
Abundant expression of granzym ...
Homo sapiens
J. Immunol.
173
641-648
2004
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
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