Literature summary for 3.4.21.76 extracted from

Kantari, C.; Millet, A.; Gabillet, J.; Hajjar, E.; Broemstrup, T.; Pluta, P.; Reuter, N.; Witko-Sarsat, V.
Molecular analysis of the membrane insertion domain of proteinase 3, the Wegeners autoantigen, in RBL cells: implication for its pathogenic activity (2011), J. Leukoc. Biol., 90, 941-950.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in RBL cell	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
F180A/F181A	mutant is still able to display membrane PR3 after degranulation, with similar fluorescence intensity as wild-type	Homo sapiens
F180A/F181A/L228A/F229A	contrary to wild-type, membrane anchorage is abrogated in mutant. Mutant is still able to cleave the synthetic substrate Boc-Ala-Pro-Val in cell lysates, but fails to cleave extracellular fibronectin, is not externalized after apoptosis and does not impair macrophage phagocytosis of apoptotic cells, does not promote myeloid cell proliferation and fails to cleave p21/waf1	Homo sapiens
F180A/L228A/F229A	mutant is still able to display membrane PR3 after degranulation, with similar fluorescence intensity as wild-type	Homo sapiens
L228A	mutant is still able to display membrane PR3 after degranulation, with similar fluorescence intensity as wild-type	Homo sapiens
L228A/F229A	mutant is still able to display membrane PR3 after degranulation, with similar fluorescence intensity as wild-type	Homo sapiens
R193A/R194A/K195A	mutant does not affect membrane expression	Homo sapiens
R193A/R194A/K195A/R227A	contrary to wild-type, membrane anchorage is abrogated in mutant	Homo sapiens
R194A/K195A/R227A	mutant does not affect membrane expression	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	hydrophobic patch F180-F181-L228-F229 is essential for PR3 membrane anchorage. PR3 membrane insertion appears to be pivotal for its proinflammatory activities, such as extracellular proteolysis and impairment of apoptotic cell clearance, but also for myeloid cell proliferation	Homo sapiens	16020	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

General Information

General Information	Comment	Organism
physiological function	PR3 membrane insertion appears to be pivotal for its proinflammatory activities, such as extracellular proteolysis and impairment of apoptotic cell clearance, but also for myeloid cell proliferation	Homo sapiens

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Release 2023.1 (January 2023)