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Literature summary for 3.4.21.76 extracted from
- Production and applications of recombinant proteinase 3, Wegeners autoantigen: problems and perspectives (2006), Clin. Nephrol., 66, 153-159.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | recombinant PR3 variants have a great potential to study individual anti-PR3 responses and will advance the development of new epitope-based therapeutics | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Sf-9 insect cells, HEK-293, HMC-1, RBL-1 and CHO cells. Expression of PR3 as a completely denatured and aggregated protein in Escherichia coli. Expression in Pichia pastoris or Saccharomyces cerevisiae | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 2.2 A resolution | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | catalytically inactive Ser195-substituted PR3 variant, eliminates quality check of the antigen preperation. Recombinant PR3 variants which only carry a single antigenic region on its surface may be used to neutralize or eliminate circulating anti-neutrophil cytoplasmic antibodies (ANCA) in patients without triggering immune complex-mediated type III reactions | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha1-antitrypsin | - |
Homo sapiens | |
| monocyte-neutrophil elastase inhibitor | - |
Homo sapiens | |
| PI-1 | - |
Homo sapiens | |
| PMSF | - |
Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | - |
Homo sapiens | - |
| granulocyte | - |
Homo sapiens | - |
| HL-60 cell | cell associated PR3 levels are lower than in neutrophils, thus sensitivity of the indirect immunofluorescence test is lower | Homo sapiens | - |
| neutrophil | - |
Homo sapiens | - |
| serum | patients with Wegeners granulomatosis show PR3-ANCA titers | Homo sapiens | - |
| U-937 cell | cell associated PR3 levels are lower than in neutrophils, thus sensitivity of the indirect immunofluorescence test is lower | Homo sapiens | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PR3 | - |
Mus musculus |
| PR3 | - |
Homo sapiens |
| proteinase 3 | - |
Mus musculus |
| proteinase 3 | - |
Homo sapiens |
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