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Literature summary for 3.4.21.75 extracted from

  • Gagliardo, B.; Kubat, N.; Faye, A.; Jaouen, M.; Durel, B.; Deschemin, J.C.; Canonne-Hergaux, F.; Sari, M.A.; Vaulont, S.
    Pro-hepcidin is unable to degrade the iron exporter ferroportin unless maturated by a furin-dependent process (2009), J. Hepatol., 50, 394-401.
    View publication on PubMed
Search for more literature for 3.4.21.75

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
decanoyl-Arg-Val-Lys-Arg-chloromethylketone furin inhibitor, pro-hepcidin activity is abolished Mus musculus
pro-hepcidin hydrolytic activity of membrane furin against the fluorescent substrate Boc-RVRR-7-amino-4-methyl-coumarin is reduced by approximately 50% in presence of 2 micromol pro-hepcidin and completely abolished in presence of 5 micromol pro-hepcidin Mus musculus
siRNA furin mRNA knockdown, pro-hepcidin activity is diminished Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 Boc-RVRR-7-amido-4-methyl-coumarin
-
 Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
J-774 cell
-
 Mus musculus
-
macrophage
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000025
-
 with Boc-RVRR-7-amido-4-methyl-coumarin as substrate Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Boc-RVRR-7-amido-4-methylcoumarin + H2O
-
 Mus musculus Boc-RVRR + 7-amino-4-methylcoumarin
-
 ?
pro-hepcidin + H2O furin processes the iron-regulatory peptide hepcidin to the bioactive mature hepcidin-25 form Mus musculus active mature hepcidin
-
 ?
pro-hepcidin + H2O furin processes the iron-regulatory peptide hepcidin to the bioactive mature hepcidin-25 form Homo sapiens active mature hepcidin
-
 ?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.002
-
-
 Mus musculus pro-hepcidin