Literature summary for 3.4.21.75 extracted from

Wang, L.; Xu, D.; Qian, M.; Yang, G.; Wu, X.
Identification and characterization of Trichoplusia ni furin truncated mutant (2006), Protein Pept. Lett., 13, 171-175.

Search for more literature for 3.4.21.75

Cloned(Commentary)

Cloned (Comment)	Organism
PCR product of the truncated mutant inserted between the EcoR1 and Not1 sites of the pFastBac1H vector, expression in Tn5 cells	Trichoplusia ni

Protein Variants

Protein Variants	Comment	Organism
additional information	truncated mutant, lacking Cys-rich repeated segments	Trichoplusia ni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00124	-	pERKTR-7-amido-4-methylcoumarin	-	Trichoplusia ni
0.029	-	Boc-RVRR-7-amido-4-methylcoumarin	-	Trichoplusia ni

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	optimal concentration of mutant furin is 0.5 mM, activity gradually reduced with increasing Ca2+ concentrations	Trichoplusia ni

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68000	-	mutant, Western blot analysis	Trichoplusia ni

Organism

Organism	UniProt	Comment	Textmining
Trichoplusia ni	Q5DNW1	-	-

Purification (Commentary)

Purification (Comment)	Organism
on Ni2+-NTA column, 64fold	Trichoplusia ni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
280	-	mutant	Trichoplusia ni
18000	-	mutant, 64fold purified	Trichoplusia ni

Storage Stability

Storage Stability	Organism
-80°C, Tris-HCl buffer, pH 8.0, 50% glycerol	Trichoplusia ni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Boc-RVRR-7-amido-4-methylcoumarin + H2O	-	Trichoplusia ni	?	-	?
pERKTR-7-amido-4-methylcoumarin + H2O	-	Trichoplusia ni	?	-	?

Synonyms

Synonyms	Comment	Organism
prohormone convertase	-	Trichoplusia ni

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	-	Trichoplusia ni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.003	-	Boc-RVRR-7-amido-4-methylcoumarin	-	Trichoplusia ni
0.008	-	pERKTR-7-amido-4-methylcoumarin	-	Trichoplusia ni

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
additional information	-	between pH 7-9, mutant has a broad pH optimum shifted toward alkalinity	Trichoplusia ni

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Release 2023.1 (January 2023)