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Literature summary for 3.4.21.74 extracted from

  • Mukherjee, A.K.
    The pro-coagulant fibrinogenolytic serine protease isoenzymes purified from Daboia russelii russelii venom coagulate the blood through factor V activation: role of glycosylation on enzymatic activity (2014), PLoS ONE, 9, e86823.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0066
-
human plasma fibrinogen pH 7.4, 37°C, isozyme RV-FVPalpha Daboia russelii russelii
0.00952
-
human plasma fibrinogen pH 7.4, 37°C, isozyme RV-FVPbeta Daboia russelii russelii
0.01
-
human plasma fibrinogen pH 7.4, 37°C, isozyme RV-FVPgamma Daboia russelii russelii
0.0105
-
human plasma fibrinogen pH 7.4, 37°C, isozyme RV-FVPdelta Daboia russelii russelii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Daboia russelii russelii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
x * 23000, about , deglycosylated isozymes, SDS-PAGE, x * 38000, isozyme RV-FVPalpha, SDS-PAGE, x * 39000, isozyme RV-FVPbeta and isozyme RV-FVPgamma, SDS-PAGE, x* 40000, isozyme RV-FVPdelta, SDS-PAGE, x * 32901, isozyme RV-FVPalpha, mass spectrometry, x * 33363, isozyme RV-FVPbeta, mass spectrometry, x * 333571, isozyme RV-FVPgamma, mass spectrometry, x* 34594, isozyme RV-FVPdelta, mass spectrometry Daboia russelii russelii
32901
-
x * 23000, about , deglycosylated isozymes, SDS-PAGE, x * 38000, isozyme RV-FVPalpha, SDS-PAGE, x * 39000, isozyme RV-FVPbeta and isozyme RV-FVPgamma, SDS-PAGE, x* 40000, isozyme RV-FVPdelta, SDS-PAGE, x * 32901, isozyme RV-FVPalpha, mass spectrometry, x * 33363, isozyme RV-FVPbeta, mass spectrometry, x * 333571, isozyme RV-FVPgamma, mass spectrometry, x* 34594, isozyme RV-FVPdelta, mass spectrometry Daboia russelii russelii
33363
-
x * 23000, about , deglycosylated isozymes, SDS-PAGE, x * 38000, isozyme RV-FVPalpha, SDS-PAGE, x * 39000, isozyme RV-FVPbeta and isozyme RV-FVPgamma, SDS-PAGE, x* 40000, isozyme RV-FVPdelta, SDS-PAGE, x * 32901, isozyme RV-FVPalpha, mass spectrometry, x * 33363, isozyme RV-FVPbeta, mass spectrometry, x * 333571, isozyme RV-FVPgamma, mass spectrometry, x* 34594, isozyme RV-FVPdelta, mass spectrometry Daboia russelii russelii
38000
-
x * 23000, about , deglycosylated isozymes, SDS-PAGE, x * 38000, isozyme RV-FVPalpha, SDS-PAGE, x * 39000, isozyme RV-FVPbeta and isozyme RV-FVPgamma, SDS-PAGE, x* 40000, isozyme RV-FVPdelta, SDS-PAGE, x * 32901, isozyme RV-FVPalpha, mass spectrometry, x * 33363, isozyme RV-FVPbeta, mass spectrometry, x * 333571, isozyme RV-FVPgamma, mass spectrometry, x* 34594, isozyme RV-FVPdelta, mass spectrometry Daboia russelii russelii
39000
-
x * 23000, about , deglycosylated isozymes, SDS-PAGE, x * 38000, isozyme RV-FVPalpha, SDS-PAGE, x * 39000, isozyme RV-FVPbeta and isozyme RV-FVPgamma, SDS-PAGE, x* 40000, isozyme RV-FVPdelta, SDS-PAGE, x * 32901, isozyme RV-FVPalpha, mass spectrometry, x * 33363, isozyme RV-FVPbeta, mass spectrometry, x * 333571, isozyme RV-FVPgamma, mass spectrometry, x* 34594, isozyme RV-FVPdelta, mass spectrometry Daboia russelii russelii
333571
-
x * 23000, about , deglycosylated isozymes, SDS-PAGE, x * 38000, isozyme RV-FVPalpha, SDS-PAGE, x * 39000, isozyme RV-FVPbeta and isozyme RV-FVPgamma, SDS-PAGE, x* 40000, isozyme RV-FVPdelta, SDS-PAGE, x * 32901, isozyme RV-FVPalpha, mass spectrometry, x * 33363, isozyme RV-FVPbeta, mass spectrometry, x * 333571, isozyme RV-FVPgamma, mass spectrometry, x* 34594, isozyme RV-FVPdelta, mass spectrometry Daboia russelii russelii

Organism

Organism UniProt Comment Textmining
Daboia russelii russelii
-
isozymes RV-FVPalpha, RV-FVPbeta, RV-FVPgamma, and RV-FVPdelta
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sugar content of isozymes of 42-44%, overview. Partial deglycosylation under denaturing conditions by PNGase F Daboia russelii russelii

Source Tissue

Source Tissue Comment Organism Textmining
venom Russell's viper venom Daboia russelii russelii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
isozyme RV-FVPa, no other protease isoenzyme, displays cytotoxicity towards MCF-7 or Colo-205 cells. 250 nM of isozyme inhibits 31.7% and 6.4% of growth of MCF-7 and Colo-205 cells, respectively, after 72 h of incubation Daboia russelii russelii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Bovine fibrinogen + H2O the fibrinogen degradation products are separated by RP-HPLC followed by mass spectrometric analysis Daboia russelii russelii ?
-
?
Bovine serum albumin + H2O
-
Daboia russelii russelii ?
-
?
casein + H2O
-
Daboia russelii russelii ?
-
?
D-Val-Leu-Lys-4-nitroanilide + H2O
-
Daboia russelii russelii D-Val-Leu-Lys + 4-nitroaniline
-
?
Fibrin + H2O
-
Daboia russelii russelii ?
-
?
human plasma fibrinogen + H2O the fibrinogen degradation products are separated by RP-HPLC followed by mass spectrometric analysis Daboia russelii russelii ?
-
?
additional information specific activity of these protease isoenzymes against various protein substrates, overview. No activity with bovine serum globulin and azocasein Daboia russelii russelii ?
-
?
N-benzoyl-Ile-Glu-Gly-Arg 4-nitroanilide + H2O
-
Daboia russelii russelii N-benzoyl-Ile-Glu-Gly-Arg + 4-nitroaniline
-
?
N-benzoyl-Phe-Val-Arg-4-nitroanilide + H2O
-
Daboia russelii russelii N-benzoyl-Phe-Val-Arg + 4-nitroaniline
-
?
Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O
-
Daboia russelii russelii Nalpha-benzoyl-L-Arg + 4-nitroaniline
-
?
Nalpha-tosyl-Gly-Pro-Arg-4-nitroanilide + H2O
-
Daboia russelii russelii Nalpha-tosyl-Gly-Pro-Arg + 4-nitrolaniline
-
?

Subunits

Subunits Comment Organism
? x * 23000, about , deglycosylated isozymes, SDS-PAGE, x * 38000, isozyme RV-FVPalpha, SDS-PAGE, x * 39000, isozyme RV-FVPbeta and isozyme RV-FVPgamma, SDS-PAGE, x* 40000, isozyme RV-FVPdelta, SDS-PAGE, x * 32901, isozyme RV-FVPalpha, mass spectrometry, x * 33363, isozyme RV-FVPbeta, mass spectrometry, x * 333571, isozyme RV-FVPgamma, mass spectrometry, x* 34594, isozyme RV-FVPdelta, mass spectrometry Daboia russelii russelii

Synonyms

Synonyms Comment Organism
RVV protease
-
Daboia russelii russelii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Daboia russelii russelii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.31
-
human plasma fibrinogen pH 7.4, 37°C, isozyme RV-FVPdelta Daboia russelii russelii
0.33
-
human plasma fibrinogen pH 7.4, 37°C, isozyme RV-FVPbeta Daboia russelii russelii
0.34
-
human plasma fibrinogen pH 7.4, 37°C, isozyme RV-FVPgamma Daboia russelii russelii
1.042
-
human plasma fibrinogen pH 7.4, 37°C, isozyme RV-FVPalpha Daboia russelii russelii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Daboia russelii russelii