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Literature summary for 3.4.21.74 extracted from
- Cloning of habutobin cDNA and antithrombotic activity of recombinant protein (2007), Biochem. Biophys. Res. Commun., 362, 899-904.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | purified Autographa californica nuclear polyhedrosis virus-r-habutobin dose-dependently increases fibrin forming activity and inhibits collagen-induced aggregation of rabbit washed platelets. Thus, recombinant habutobin produced by baculoviral system is very useful for study on structure-function relationship, which is necessary for developing an antithrombotic drug from habutobin | Protobothrops flavoviridis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pET-r-habutobin expressed in inclusion body of Escherichia coli. Autographa californica nuclear polyhedrosis virus and the habutobin/pPSC12 plasmid co-transfected into Sf9 cells | Protobothrops flavoviridis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25700 | - |
- |
Protobothrops flavoviridis |
| 28000 | - |
1 * 28000, SDS-PAGE, Autographa californica nuclear polyhedrosis virus-r-habutobin | Protobothrops flavoviridis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Protobothrops flavoviridis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| His-tagged recombinant habutobin fusion protein pET-r-habutobin and Autographa californica nuclear polyhedrosis virus-r-habutobin, purified by bacterial system and baculoviral system. PET-r-habutobin purified on Ni2+-NTA column | Protobothrops flavoviridis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Protobothrops flavoviridis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Fibrinogen + H2O | - |
Protobothrops flavoviridis | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 28000, SDS-PAGE, Autographa californica nuclear polyhedrosis virus-r-habutobin | Protobothrops flavoviridis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Habutobin | - |
Protobothrops flavoviridis |
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Release 2023.1 (January 2023)
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