BRENDA - Enzyme Database
show all sequences of 3.4.21.74

Ancrod, the coagulating enzyme from Malayan pit viper (Agkistrodon rhodostoma) venom

Nolan, C.; Hall, L.S.; Barlow, G.H.; Methods Enzymol. 45, 205-213 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
4-Nitrophenyl-4-guanidinobenzoate
-
Calloselasma rhodostoma
agmatine
-
Calloselasma rhodostoma
Alpha-macroglobulin
-
Calloselasma rhodostoma
alpha-N-(4-Nitrobenzyloxycarbonyl)-Arg chloromethyl ketone
-
Calloselasma rhodostoma
antithrombin III
-
Calloselasma rhodostoma
arginine
-
Calloselasma rhodostoma
diisopropyl fluorophosphate
-
Calloselasma rhodostoma
guanidinium ions
-
Calloselasma rhodostoma
additional information
no inhibition by Nalpha-p-tosyl-L-lysine chloromethyl ketone
Calloselasma rhodostoma
PMSF
-
Calloselasma rhodostoma
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Calloselasma rhodostoma
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
amino acid composition
Calloselasma rhodostoma
35400
-
Agkistrodon rhodostoma, sedimentation equilibrium
Calloselasma rhodostoma
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Calloselasma rhodostoma
-
Malayan pit viper, formerly EC 3.4.21.28
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
high carbohydrate content
Calloselasma rhodostoma
Purification (Commentary)
Commentary
Organism
affinity chromatography, several forms, separable by electrophoresis and isoelectric focusing
Calloselasma rhodostoma
Source Tissue
Source Tissue
Commentary
Organism
Textmining
venom
-
Calloselasma rhodostoma
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
33.7
-
tosyl-L-Arg methyl ester
Calloselasma rhodostoma
Storage Stability
Storage Stability
Organism
5C, pH 5-6.8, several months
Calloselasma rhodostoma
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Fibrinogen + H2O
splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain
29684
Calloselasma rhodostoma
?
-
-
-
-
Fibrinogen + H2O
or gamma-chain
29684
Calloselasma rhodostoma
?
-
-
-
-
additional information
no activation of human factor VIII
29684
Calloselasma rhodostoma
?
-
-
-
-
additional information
Ancrod has a high degree of specificity for Arg esters and peptide bonds involving carboxyl group of Arg residues
29684
Calloselasma rhodostoma
?
-
-
-
-
additional information
no hydrolysis of native casein, denatured hemoglobin, Nalpha-benzoyl-Arg ethyl ester
29684
Calloselasma rhodostoma
?
-
-
-
-
Nalpha-Benzoyl-L-Arg cyclohexyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + cyclohexanol
-
-
-
-
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + ethanol
-
-
-
-
Nalpha-Benzoyl-L-Arg methyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + methanol
-
-
-
-
Tosyl-L-arginine methyl ester + H2O
-
29684
Calloselasma rhodostoma
Tosyl-L-arginine + methanol
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Calloselasma rhodostoma
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
6.8
several months stable at 5C
Calloselasma rhodostoma
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-Nitrophenyl-4-guanidinobenzoate
-
Calloselasma rhodostoma
agmatine
-
Calloselasma rhodostoma
Alpha-macroglobulin
-
Calloselasma rhodostoma
alpha-N-(4-Nitrobenzyloxycarbonyl)-Arg chloromethyl ketone
-
Calloselasma rhodostoma
antithrombin III
-
Calloselasma rhodostoma
arginine
-
Calloselasma rhodostoma
diisopropyl fluorophosphate
-
Calloselasma rhodostoma
guanidinium ions
-
Calloselasma rhodostoma
additional information
no inhibition by Nalpha-p-tosyl-L-lysine chloromethyl ketone
Calloselasma rhodostoma
PMSF
-
Calloselasma rhodostoma
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Calloselasma rhodostoma
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
additional information
-
amino acid composition
Calloselasma rhodostoma
35400
-
Agkistrodon rhodostoma, sedimentation equilibrium
Calloselasma rhodostoma
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
high carbohydrate content
Calloselasma rhodostoma
Purification (Commentary) (protein specific)
Commentary
Organism
affinity chromatography, several forms, separable by electrophoresis and isoelectric focusing
Calloselasma rhodostoma
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
venom
-
Calloselasma rhodostoma
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
33.7
-
tosyl-L-Arg methyl ester
Calloselasma rhodostoma
Storage Stability (protein specific)
Storage Stability
Organism
5C, pH 5-6.8, several months
Calloselasma rhodostoma
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Fibrinogen + H2O
splits fibrinopeptide A from Aalpha-chains by hydrolysis of Arg-Gly bond, but does not split this bond in Bbeta-chain
29684
Calloselasma rhodostoma
?
-
-
-
-
Fibrinogen + H2O
or gamma-chain
29684
Calloselasma rhodostoma
?
-
-
-
-
additional information
no activation of human factor VIII
29684
Calloselasma rhodostoma
?
-
-
-
-
additional information
Ancrod has a high degree of specificity for Arg esters and peptide bonds involving carboxyl group of Arg residues
29684
Calloselasma rhodostoma
?
-
-
-
-
additional information
no hydrolysis of native casein, denatured hemoglobin, Nalpha-benzoyl-Arg ethyl ester
29684
Calloselasma rhodostoma
?
-
-
-
-
Nalpha-Benzoyl-L-Arg cyclohexyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + cyclohexanol
-
-
-
-
Nalpha-Benzoyl-L-Arg ethyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + ethanol
-
-
-
-
Nalpha-Benzoyl-L-Arg methyl ester + H2O
-
29684
Calloselasma rhodostoma
Nalpha-Benzoyl-L-Arg + methanol
-
-
-
-
Tosyl-L-arginine methyl ester + H2O
-
29684
Calloselasma rhodostoma
Tosyl-L-arginine + methanol
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Calloselasma rhodostoma
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
6.8
several months stable at 5C
Calloselasma rhodostoma
Other publictions for EC 3.4.21.74
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731423
Zaqueo
Isolation and biochemical char ...
Bothrops pirajai
BioMed Res. Int.
2014
595186
2014
-
-
-
-
-
-
2
-
1
-
2
1
-
4
-
1
1
-
-
3
-
-
7
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
2
1
-
-
1
1
-
3
-
-
7
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
732778
Mukherjee
The pro-coagulant fibrinogenol ...
Daboia russelii russelii
PLoS ONE
9
e86823
2014
-
-
-
-
-
-
-
4
1
-
6
-
-
6
-
1
-
-
-
1
1
-
11
1
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
1
-
6
-
-
-
1
-
-
1
1
-
11
1
1
-
-
4
1
-
-
-
-
-
-
-
-
-
731530
He
TA-2, a thrombin-like enzyme f ...
Trimeresurus albolabris
Blood Coagul. Fibrinolysis
23
445-453
2012
-
-
-
-
-
-
-
-
1
-
2
-
-
3
-
1
1
-
-
1
2
-
5
2
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
1
-
1
2
-
5
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
717128
Jiang
Soluble expression, purificati ...
Gloydius shedaoensis
Appl. Microbiol. Biotechnol.
85
635-642
2010
-
-
1
-
-
-
3
-
3
-
1
-
-
4
-
-
1
-
-
3
1
-
3
1
1
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
3
-
1
-
-
-
-
1
-
3
1
-
3
1
1
1
-
-
1
1
-
-
-
1
1
-
-
-
718430
Costa
Bhalternin: Functional and str ...
Bothrops alternatus
Toxicon
55
1365-1377
2010
-
1
1
-
-
-
1
-
1
-
2
1
-
5
-
-
1
-
-
3
-
-
4
1
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
1
-
-
1
-
2
1
-
-
-
1
-
3
-
-
4
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
699848
Levy
Ancrod for acute ischemic stro ...
synthetic construct
J. Stroke Cerebrovasc. Dis.
18
23-27
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
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1
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-
-
-
-
-
-
-
679349
Giron
Hemorrhagic, coagulant and fib ...
Bothrops colombiensis
Comp. Biochem. Physiol. C
147C
113-121
2008
-
1
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
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-
1
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1
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-
-
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-
1
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1
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-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701326
Mukherjee
Characterization of a novel pr ...
Daboia russelii russelii
Toxicon
51
923-933
2008
-
-
-
-
-
-
4
-
-
6
3
-
-
5
-
-
1
-
-
2
2
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
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-
6
3
-
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-
1
-
2
2
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
677981
Sunagawa
Cloning of habutobin cDNA and ...
Protobothrops flavoviridis
Biochem. Biophys. Res. Commun.
362
899-904
2007
-
1
1
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
2
-
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1
1
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-
1
1
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2
-
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-
1
-
2
-
-
1
1
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-
-
-
-
-
-
-
-
-
-
-
-
682676
Yu
Expression and purification of ...
Calloselasma rhodostoma
Protein Expr. Purif.
55
257-261
2007
-
1
1
-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
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-
1
1
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-
-
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-
1
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1
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
682928
Muanpasitporn
Expression and characterizatio ...
Trimeresurus albolabris
Toxicon
49
1083-1089
2007
-
1
1
-
-
-
-
-
-
-
2
-
-
5
-
-
1
-
-
1
-
-
4
1
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-
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-
-
-
-
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-
1
1
-
-
-
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-
-
2
-
-
-
-
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
653973
Siigur
Anticoagulant serine fibrinoge ...
Macrovipera lebetina
Thromb. Haemost.
89
826-831
2003
-
-
1
-
-
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
-
-
1
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-
-
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1
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-
1
-
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1
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1
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-
1
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
649172
Watanabe
Crystallization of bothrombin, ...
Bothrops jararaca
Acta Crystallogr. Sect. D
58
1036-1038
2002
-
-
-
1
-
-
-
-
-
-
-
1
-
3
-
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1
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1
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1
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1
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1
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1
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-
-
-
-
-
-
-
-
-
651434
Siigur
Proteases from Vipera lebetina ...
Macrovipera lebetina
Haemostasis
31
123-132
2001
-
-
-
-
-
-
-
-
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2
1
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3
-
-
1
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2
1
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2
1
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1
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2
1
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650723
Dempfle
Analysis of fibrin formation a ...
Calloselasma rhodostoma
Blood
96
2793-2802
2000
-
1
-
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-
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1
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2
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1
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1
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2
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1
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1
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1
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1
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2
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-
-
29680
Hung
Isolation of multiple isoforms ...
Crotalus atrox
Biochem. Biophys. Res. Commun.
201
1414-1423
1994
-
-
-
-
-
-
4
-
1
1
4
1
-
4
-
-
1
-
-
1
-
-
5
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
1
4
1
-
-
-
1
-
1
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5
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
29683
Nakamura
Production of a monoclonal ant ...
Protobothrops flavoviridis
Toxicon
30
1177-1188
1992
-
-
-
-
-
-
-
-
-
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6
-
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-
3
-
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3
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29681
Itoh
Organization of the gene for b ...
Bothrops atrox, Bothrops moojeni
J. Biol. Chem.
263
7628-7631
1988
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1
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-
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-
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-
-
3
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29678
Simmons
Action of crotalase, an enzyme ...
Crotalus adamanteus
Thromb. Res.
40
555-561
1985
-
-
-
-
-
-
2
10
-
-
-
-
-
1
-
-
-
-
-
1
-
-
18
-
1
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
-
1
-
-
18
-
1
-
-
12
-
-
-
-
-
-
-
-
-
-
29679
Markland
Kallikrein-like activity of cr ...
Crotalus adamanteus
Proc. Natl. Acad. Sci. USA
79
1688-1692
1982
-
-
-
-
-
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1
3
-
-
1
-
-
7
-
-
-
-
-
8
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
-
8
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
29682
Stocker
The coagulant enzyme from Both ...
Bothrops asper, Bothrops atrox, Bothrops marajoensis, Bothrops moojeni
Methods Enzymol.
45
214-223
1976
8
-
-
-
-
2
12
-
4
-
11
-
-
4
-
3
3
-
-
4
4
2
39
3
4
-
2
-
4
-
2
-
-
-
-
8
-
-
-
-
-
2
-
12
-
-
4
-
11
-
-
-
3
3
-
4
4
2
39
3
4
-
2
-
4
-
2
-
-
-
-
-
-
-
29684
Nolan
Ancrod, the coagulating enzyme ...
Calloselasma rhodostoma
Methods Enzymol.
45
205-213
1976
-
-
-
-
-
-
10
-
1
-
2
-
-
2
-
1
1
-
-
2
1
1
9
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
-
1
-
2
-
-
-
1
1
-
2
1
1
9
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-