Protein Variants | Comment | Organism |
---|---|---|
L97bG/H99Y/S195A/R217E | site-directed mutagenesis, the mutant shows altered interaction with human plasminogen activator inhibitor-1 compare to wild-type | Homo sapiens |
L97bG/S195A | site-directed mutagenesis, the mutant shows altered interaction with human plasminogen activator inhibitor-1 compare to wild-type | Homo sapiens |
L97bG/S195A/R217E | site-directed mutagenesis, the mutant shows altered interaction with human plasminogen activator inhibitor-1 compare to wild-type | Homo sapiens |
additional information | analysis of interaction kinetics of the wild-type and mutant human enzymes with human and zebrafish plasminogen activator inhibitor-1 and of the zebrafish wild-type and mutant enzyme with both inhibitors, overview | Homo sapiens |
S195A | site-directed mutagenesis, the mutant shows altered interaction with human plasminogen activator inhibitor-1 compare to wild-type | Homo sapiens |
S195A/R217E | site-directed mutagenesis, the mutant shows altered interaction with human plasminogen activator inhibitor-1 compare to wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
human PAI-1 | human plasminogen activator inhibitor-1, complex structure of uPA:PAI-1 Michaelis complex, interaction analysis, the S3-pocket-lining residues of uPA and the P3 residue of both PAI-1 and plasminogen form numerous polar interactions in the human uPA:PAI-1 Michaelis complex, overview | Homo sapiens | |
additional information | although the interaction areas between protease-substrate and protease-inhibitor are shared, the two interactions are mechanistically different. Species specificity of the reaction of PAI-1 and uPA. Analysis of interaction kinetics of the wild-type and mutant human enzymes with human and zebrafish plasminogen activator inhibitor-1 and of the zebrafish wild-type and mutant enzyme with both inhibitors, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00749 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
uPA | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | biochemical and structural analyses suggest that plasminogen activators coevolved with their cognate protein substrates and inhibitors. The binding interfaces of uPA:plasminogen and uPA:PAI-1 may have coevolved to maintain tight interactions | Homo sapiens |
additional information | although the interaction areas between protease-substrate and protease-inhibitor are shared, the two interactions are mechanistically different | Homo sapiens |