Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl phosphofluoridate | - |
Homo sapiens | |
phenylmethanesulfonyl fluoride | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04706 | - |
Benzyloxycarbonylalanine 4-nitrophenyl ester | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
1 * 28000, human, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
22°C, 50 mM Tris/100 mM NaCl, pH 7.9, 15 mM NaN3, with or without 10 mM CaCl2, loss of approximately 8% of its esterolytic activity per week | Homo sapiens |
4°C, stable over months without loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Benzyloxycarbonylalanine 4-nitrophenyl ester + H2O | - |
Homo sapiens | Benzyloxycarbonylalanine + 4-nitrophenol | - |
? | |
Chymotrypsin + H2O | - |
Homo sapiens | Hydrolyzed chymotrypsin | - |
? | |
Elastase + H2O | - |
Homo sapiens | Hydrolyzd elastase | - |
? | |
Glucagon + H2O | carbonyl bond of Ala is the favoured cleavage site | Homo sapiens | ? | - |
? | |
additional information | chromogenic substrates | Homo sapiens | ? | - |
? | |
Trypsin + H2O | - |
Homo sapiens | Hydrolyzed trypsin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, human, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
41 | - |
cleavage of benzyloxycarbonylalanine 4-nitrophenyl ester | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
56 | - |
loss of activity within 5 min | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | - |
benzyloxycarbonylalanine 4-nitrophenyl ester | Homo sapiens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
relatively resistant towards acid, the activity decreases by 20% by incubation in 50 mM acetic acid and by 60% in 50 mM HCl, complete inactivation by treatment with 50 mM NaOH | Homo sapiens |