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Literature summary for 3.4.21.7 extracted from

  • Robbins, K.C.; Summaria, L.
    Human plasminogen and plasmin (1970), Methods Enzymol., 19, 184-199.
No PubMed abstract available
Search for more literature for 3.4.21.7

General Stability

General Stability Organism
freezing and thawing results in loss of activity Homo sapiens
reduction with 2-mercaptoethanol in 8 M urea at pH 9.0, complete loss of activity by cleavage of a single disulfide bond Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
6-aminohexanoic acid noncompetitive Homo sapiens
alpha1 globulin
-
 Homo sapiens
alpha2 globulin
-
 Homo sapiens
aminomethyl cyclohexane carboxylic acid
-
 Homo sapiens
human plasma protein inhibitors
-
 Homo sapiens
Lima bean trypsin inhibitor
-
 Homo sapiens
Pancreatic trypsin inhibitor
-
 Homo sapiens
Soybean trypsin inhibitor
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 p-tosyl-Arg methyl ester
-
 Homo sapiens
0.03
-
 fibrinogen
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75400
-
 equilibrium sedimentation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification mechanism of activation by urokinase, streptokinase, trypsin and pig heart activator is specific and proceeds primarily through the cleavage of a single Arg-Val bond Homo sapiens
proteolytic modification zymogen: plasminogen Homo sapiens
side-chain modification the enzyme contains less than 2% carbohydrate Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-20°C to -30°C, pH 9.0, Tris-Lys-NaCl-EDTA buffer containing 25% glycerol, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Homo sapiens ?
-
 ?
insulin + H2O cleavage of the Arg25-Gly and Lys29-Ala peptide bonds of the beta-chain of oxidized bovine insulin Homo sapiens ?
-
 ?
protamin-heparin complex + H2O
-
 Homo sapiens ?
-
 ?
S-sulfo-fibrinogen + H2O cleaves only Lys and Arg peptide bonds Homo sapiens ?
-
 ?
Tosyl-Arg methyl ester + H2O p-tosyl-Arg methyl ester Homo sapiens ?
-
 ?

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 very stable below Homo sapiens
7
-
 unstable in absence of glycerol Homo sapiens