Application | Comment | Organism |
---|---|---|
pharmacology | construction of a deletion mutant of plasminogen lacking the middle portion of the molecule, resulting in kringle I attachment to the serine protease domain. After expression in Escherichia coli and purification, mutant plasminogen is effectively converted to mutant plasmin by tissue plasminogen activator. Mutant plasmin is rapidly inhibited by alpha2-antiplasmin an alpha2-macroglobulin. It demonstrates fibrinolytic potency comparable to human plasma-derived plasmin and is a potentially safe and effective direct thrombolytic agent | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a deletion mutant of plasminogen lacking the middle portion of the molecule, resulting in kringle I attachment to the serine protease domain. After expression in Escherichia coli and purification, mutant plasminogen is effectively converted to mutant plasmin by tissue plasminogen activator. Mutant plasmin is rapidly inhibited by alpha2-antiplasmin an alpha2-macroglobulin. It demonstrates fibrinolytic potency comparable to human plasma-derived plasmin | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha2-antiplasmin | similar inhibition of native enzyme and deletion mutant lacking the middle portion of the molecule | Homo sapiens | |
alpha2-Macroglobulin | similar inhibition 0f native enzyme and deletion mutant lacking the middle portion of the molecule | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.138 | - |
D-Val-L-Leu-L-Lys-4-nitroanilide | deletion mutant lacking the middle portion of the protein | Homo sapiens | |
0.193 | - |
D-Val-L-Leu-L-Lys-4-nitroanilide | native enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Val-L-Leu-L-Lys-4-nitroanilide + H2O | i.e. S2251 | Homo sapiens | D-Val-L-Leu-L-Lys + 4-nitroaniline | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.6 | - |
D-Val-L-Leu-L-Lys-4-nitroanilide | deletion mutant lacking the middle portion of the protein | Homo sapiens | |
12.7 | - |
D-Val-L-Leu-L-Lys-4-nitroanilide | native enzyme | Homo sapiens |