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Literature summary for 3.4.21.7 extracted from

  • Hunt, J.A.; Petteway, S.R.; Scuderi, P.; Novokhatny, V.
    Simplified recombinant plasmin: production and functional comparison of a novel thrombolytic molecule with plasma-derived plasmin (2008), Thromb. Haemost., 100, 413-419.
    View publication on PubMed

Application

Application Comment Organism
pharmacology construction of a deletion mutant of plasminogen lacking the middle portion of the molecule, resulting in kringle I attachment to the serine protease domain. After expression in Escherichia coli and purification, mutant plasminogen is effectively converted to mutant plasmin by tissue plasminogen activator. Mutant plasmin is rapidly inhibited by alpha2-antiplasmin an alpha2-macroglobulin. It demonstrates fibrinolytic potency comparable to human plasma-derived plasmin and is a potentially safe and effective direct thrombolytic agent Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of a deletion mutant of plasminogen lacking the middle portion of the molecule, resulting in kringle I attachment to the serine protease domain. After expression in Escherichia coli and purification, mutant plasminogen is effectively converted to mutant plasmin by tissue plasminogen activator. Mutant plasmin is rapidly inhibited by alpha2-antiplasmin an alpha2-macroglobulin. It demonstrates fibrinolytic potency comparable to human plasma-derived plasmin Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
alpha2-antiplasmin similar inhibition of native enzyme and deletion mutant lacking the middle portion of the molecule Homo sapiens
alpha2-Macroglobulin similar inhibition 0f native enzyme and deletion mutant lacking the middle portion of the molecule Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.138
-
D-Val-L-Leu-L-Lys-4-nitroanilide deletion mutant lacking the middle portion of the protein Homo sapiens
0.193
-
D-Val-L-Leu-L-Lys-4-nitroanilide native enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Val-L-Leu-L-Lys-4-nitroanilide + H2O i.e. S2251 Homo sapiens D-Val-L-Leu-L-Lys + 4-nitroaniline
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.6
-
D-Val-L-Leu-L-Lys-4-nitroanilide deletion mutant lacking the middle portion of the protein Homo sapiens
12.7
-
D-Val-L-Leu-L-Lys-4-nitroanilide native enzyme Homo sapiens