Literature summary for 3.4.21.7 extracted from

Tanka-Salamon, A.; Tenekedjiev, K.; Machovich, R.; Kolev, K.
Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids. Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo stimulation (2008), FEBS J., 275, 1274-1282.

Search for more literature for 3.4.21.7

Activating Compound

Activating Compound	Comment	Organism	Structure
arachidonate	-	synthetic construct
oleate	-	synthetic construct
stearate	-	synthetic construct

Protein Variants

Protein Variants	Comment	Organism
additional information	truncated plasmin variants. Miniplasmin (des-kringle 1-4 plasmin) contains the kringle 5 and the catalytic domain of plasmin, whereas microplasmin (des-kringle 1-5 plasmin) is composed of the catalytic domain only. Miniplasmin (des-kringle 1-4 plasmin) is as sensitive to fatty acids as plasmin, whereas the activity of microplasmin (des-kringle 1-5 plasmin) is not affected	synthetic construct

Inhibitors

Inhibitors	Comment	Organism	Structure
arachidonate	-	synthetic construct
oleate	-	synthetic construct
stearate	-	synthetic construct

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00589	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the absence of fatty acids	synthetic construct
0.00817	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.065 mM stearate	synthetic construct
0.01133	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.115 mM stearate	synthetic construct
0.01258	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.01 mM oleate	synthetic construct
0.02009	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.025 mM oleate	synthetic construct
0.02337	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.175 mM stearate	synthetic construct
0.02371	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.01 mM arachidonate	synthetic construct
0.02749	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.045 mM oleate	synthetic construct
0.04265	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.025 mM arachidonate	synthetic construct
0.05751	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.045 mM arachidonate	synthetic construct
0.05985	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.065 mM arachidonate	synthetic construct
0.07296	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.23 mM stearate	synthetic construct
0.1311	-	D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide	in the presence of 0.065 mM oleate	synthetic construct

Organism

Organism	UniProt	Comment	Textmining
synthetic construct	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide + H2O	-	synthetic construct	?	-	?

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0002	-	stearate	in the presence of 0.23 mM stearate	synthetic construct
0.0007	-	stearate	in the presence of 0.175 mM stearate	synthetic construct
0.0012	-	stearate	in the presence of 0.115 mM stearate	synthetic construct
0.0025	-	stearate	in the presence of 0.065 mM stearate	synthetic construct

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Release 2023.1 (January 2023)