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Literature summary for 3.4.21.69 extracted from

  • Sen, P.; Sahoo, S.; Pendurthi, U.R.; Rao, L.V.
    Zinc modulates the interaction of protein C and activated protein C with endothelial cell protein C receptor (2010), J. Biol. Chem., 285, 20410-20420.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.69

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ Zn2+ enhances the binding of protein C/activated protein C to endothelial cell protein C receptor on endothelial cells by increasing the binding affinities. Zn2+ binding induces conformational changes in protein C/APC. Zn2+ binding to APC inhibits the amidolytic activity of APC, but the inhibition is reversed by Ca2+. Zn2+ increases the rate of APC generation on endothelial cells in the presence of physiological concentrations of Ca2+, but does not further enhance increased APC generation obtained in the presence of physiological concentrations of Mg2+ with Ca2+. Zn2+ has no effect on the anticoagulant activity of APC. Zn2+-mediated inhibition of APC amidolytic activity rather than the direct inhibition of protein C activation Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the presence of Ca2+ is essential for Zn2+ to enhance protein C activation because Zn2+ alone fails to support protein C activation Homo sapiens
Zn2+ Zn2+ enhances the binding of protein C/activated protein C to endothelial cell protein C receptor on endothelial cells by increasing the binding affinities. Zn2+ binding induces conformational changes in protein C/APC. Zn2+ binding to APC inhibits the amidolytic activity of APC, but the inhibition is reversed by Ca2+. Zn2+ increases the rate of APC generation on endothelial cells in the presence of physiological concentrations of Ca2+, but does not further enhance increased APC generation obtained in the presence of physiological concentrations of Mg2+ with Ca2+. Zn2+ has no effect on the anticoagulant activity of APC. Zn2+ enhances APC-mediated activation of protease activated receptor 1 and p44/42 MAPK Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Factor Va + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Homo sapiens
-
endothelial cell primary human umbilical vein endothelial cells, HUVEC Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Factor Va + H2O
-
 Homo sapiens ?
-
 ?
pyroGlu-Pro-Arg-4-nitroanilide + H2O S-2366, APC is labeled with AF546 Homo sapiens pyroGlu-Pro-Arg + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
Activated protein C
-
 Homo sapiens
APC
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

General Information

General Information Comment Organism
metabolism the protein C pathway plays a key role in blood coagulation and inflammation Homo sapiens
additional information APC generation is dependent on protein C binding to endothelial cell protein C receptor, EPCR, and thrombin binding to thrombomodulin Homo sapiens