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Literature summary for 3.4.21.69 extracted from

  • Norstrom, E.A.; Tran, S.; Steen, M.; Dahlbaeck, B.
    Effects of factor Xa and protein S on the individual activated protein C-mediated cleavages of coagulation factor Va (2006), J. Biol. Chem., 281, 31486-31494.
    View publication on PubMed
Search for more literature for 3.4.21.69

Activating Compound

Activating Compound Comment Organism Structure
factor Xa stimulates factor Va cleavage at Arg306, but inhibits the cleavage at Arg506 20fold, overview, factor Xa and protein S interact with distinct sites on factor Va Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
factor Xa stimulates factor Va cleavage at Arg306, but inhibits the cleavage at Arg506 20fold, which is counteracted by protein S, overview, factor Xa and protein S interact with distinct sites on factor Va Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
active factor Va + H2O Homo sapiens activated protein C inhibits the procoagulant function of activated factor V through proteolytic cleavage inactive factor V + domain B
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
active factor Va + H2O activated protein C inhibits the procoagulant function of activated factor V through proteolytic cleavage Homo sapiens inactive factor V + domain B
-
 ?
active factor Va + H2O proteolytic inactivation, cleavages at Arg306, Arg506, and Arg679, the cleavage at Arg506 is kinetically favored but protected by factor Xa Homo sapiens inactive factor V + domain B
-
 ?
active factor Va mutant R306Q/R679Q + H2O recombinant substrate expressed in COS-1 cells, proteolytic inactivation, cleavage of the heavy chain at Arg506, protected by factor Xa Homo sapiens inactive factor V mutant R306Q/R679Q + ?
-
 ?
active factor Va mutant R506Q/R679Q + H2O recombinant substrate expressed in COS-1 cells, proteolytic inactivation, cleavage of the heavy chain at Arg306, stimulated by factor Xa Homo sapiens inactive factor V mutant R506Q/R679Q + ?
-
 ?
S2238 + H2O a commercial chromogenic substrate Homo sapiens ?
-
 ?
S2366 + H2O a commercial chromogenic substrate Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
Activated protein C
-
 Homo sapiens
APC
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
protein S inhibits the inhibition of factor Va cleavage by factor Xa, factor Xa and protein S interact with distinct sites on FVa Homo sapiens