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Literature summary for 3.4.21.69 extracted from

  • Nicolaes, G.A.F.; Tans, G.; Thomassen, C.L.G.D.; Hemker, H.C.; Pabinger, I.; Varadi, K.; Schwarz, H.P.; Rosing, J.
    Peptide bond cleavages and loss of functional activity during inactivation of factor Va and factor VaR506Q by activated protein C (1995), J. Biol. Chem., 270, 21158-21166.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Phospholipid accelerates cleavages at Arg506, Arg679 and Arg306 to a different extent Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002
-
membrane-bound factor Va
-
Homo sapiens
0.000196
-
Factor VaR506Q
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Factor Va + H2O in the membrane-bound factor Va cleavage occurs rapidly at Arg506 and is followed by a slower proteolysis at Arg306 and a final, very slow cleavage at Arg679 Homo sapiens ?
-
?
Factor VaR506Q + H2O The substrate is derived from factor Va by replacing Arg506 by Gln. In the presence of phospholipids the substrate is hydrolyzed at Arg306, but in the absence of phospholipids hydrolysis occurs at Arg679, followed by cleavage at Arg306, suggesting that the binding of phospholipids alters the accessibility of Arg679 Homo sapiens ?
-
?
Factor VaR506Q + H2O cleavage of Arg306 in factor VaR506Q Homo sapiens ?
-
?
membrane-bound factor Va + H2O cleavage of Arg506 in membrane-bound factor Va Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
Factor VaR506Q
-
Homo sapiens
0.96
-
membrane-bound factor Va
-
Homo sapiens