Literature summary for 3.4.21.68 extracted from

Lee, S.Y.; Kim, H.J.; Lee, W.J.; Joo, S.H.; Jeon, S.J.; Kim, J.W.; Kim, H.S.; Han, S.H.; Lee, J.; Park, S.H.; Cheong, J.H.; Kim, W.K.; Ko, K.H.; Shin, C.Y.
Differential regulation of matrix metalloproteinase-9 and tissue plasminogen activator activity by the cyclic-AMP system in lipopolysaccharide-stimulated rat primary astrocytes (2008), Neurochem. Res., 33, 2324-2334.

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Activating Compound

Activating Compound	Comment	Organism	Structure
dibutyryl-cAMP	slightly increases level of tPA mRNA expression. Concentration-dependently increases tPA activity in both basal and lipopolysaccharide-stimulated rat primary astrocytes. Treatment with 100 micromol increases tPA activity by 757.9% compared with lipopolysaccharide treatment. Differentially modulates MMP-9 and tPA activity through a mechanism related to PKA activation. Co-treatment with lipopolysaccharide and dibutyryl-cAMP does not have an additive effect on tPA mRNA induction	Rattus norvegicus
IBMX	increases tPA activity in lipopolysaccharide-stimulated rat primary astrocytes	Rattus norvegicus
lipopolysaccharide	slightly increases level of tPA mRNA expression. Co-treatment with lipopolysaccharide and dibutyryl-cAMP does not have an additive effect on tPA mRNA induction	Rattus norvegicus
rolipram	increases tPA activity in lipopolysaccharide-stimulated rat primary astrocytes	Rattus norvegicus

Cloned(Commentary)

Cloned (Comment)	Organism
astrocytes co-transfected with metalloproteinase-9 or tPA luciferase reporter plasmid and pCMV-beta-galactosidase reporter plasmid	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism	Structure
H89	inhibits dibutyryl-cAMP-mediated changes in tPA activity without affecting metalloproteinase-9 activity	Rattus norvegicus
lipopolysaccharide	decreases tPA activity. Co-treatment with dibutyryl-cAMP and lipopolysaccharide dose-dependently prevents lipopolysaccharide-induced downregulation of tPA activity	Rattus norvegicus
plasminogen activator inhibitor-1	plays a major role in the regulation of tPA activity	Rattus norvegicus
Rp-cAMP	inhibits dibutyryl-cAMP-mediated changes in tPA activity without affecting metalloproteinase-9 activity	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68000	-	casein zymography or gel filtration	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	Sprague-Dawley rats	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	primary astrocyte	Rattus norvegicus	-
pup	1- to 2-day-old pups	Rattus norvegicus	-

Synonyms

Synonyms	Comment	Organism
Tissue plasminogen activator	-	Rattus norvegicus
tPA	-	Rattus norvegicus

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Release 2023.1 (January 2023)