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Literature summary for 3.4.21.68 extracted from

  • Hu, C.K.; Kohnert, U.; Wilhelm, O.; Fischer, S.; Llinas, M.
    Tissue-type plasminogen activator domain-deletion mutant BM 06.022: modular stability, inhibitor binding, and activation cleavage (1994), Biochemistry, 33, 11760-11766.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,5-dansyl-L-glutamylglycyl-L-arginine chloromethyl ketone
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39590
-
human, recombinant domain-deletion mutant BM 06.022, calculation from amino acid sequence Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant domain-deletion mutant BM 06.022
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plasminogen + H2O
-
Homo sapiens plasmin + ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
two distinct thermal melting transitions, at about 334 K and 252 K, assigned to an irreversible denaturation of the protease and a reversible unfolding of the kringle 2, respectively Homo sapiens
additional information
-
enzyme having reacted with the inhibitor 1,5-dansyl-L-glutamylglycyl-L-arginine chloromethyl ketone has an about 10 K higher thermal stability than the inhibitor-free protease Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.8
-
reversible acid/base unfolding below Homo sapiens