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Literature summary for 3.4.21.66 extracted from
- Catalytic, thermodynamic and structural properties of an immobilized and highly thermostable alkaline protease from a haloalkaliphilic actinobacteria, Nocardiopsis alba TATA-5 (2019), Biores. Technol., 278, 150-158 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| yes | purifed enzyme is immobilized on different carriers (chitin, chitosan, alumina, ceramic, PVC, silica gel, and polystyrene), method evaluation, overview. Enzyme entrapment in polyacrylamide, calcium alginate, and alginate and glutaraldehyde activated chitosan. Best immobilization yield and recovery activity is obtained on silica gels. Immobilization of the alkaline protease from Nocardiopsis alba Tata-5 by ionic binding | Nocardiopsis alba |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Lineweaver-Burk plots of the free and immobilized protease provide Km (Michaelis constant) of 0.01 mg/ml with casein, which is lower than that of the immobilized protease on chitosan (0.015 mg/ml), DEAE Sephadex A50 (0.012 mg/ml), silica gel 100-200 (0.014 mg/ml), polyacrylamide (0.011 mg/ml) and nitrocellulose membrane (0.011 mg/ml) | Nocardiopsis alba |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nocardiopsis alba | - |
- |
- |
| Nocardiopsis alba TATA-5 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from Nocardia alba strain TATA-5 by ammonium sulfate fractionation and anion exchange chromatography | Nocardiopsis alba |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Nocardiopsis alba | ? | - |
? |  |
| casein + H2O | - |
Nocardiopsis alba TATA-5 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alkaline protease | - |
Nocardiopsis alba |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
free enzyme | Nocardiopsis alba |
| 70 | - |
immobilized enzyme on chitosan, silica gel 100-200, alginate and glutaraldehyde activated chitosan and nitrocellulose membrane | Nocardiopsis alba |
| 80 | - |
immobilized enzyme on amberlite IR 120, DEAE Sephadex A50, and polyacrylamide | Nocardiopsis alba |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
immobilized enzyme on chitosan, amberlite IR 120, DEAE Sephadex A50, alginate and glutaraldehyde activated chitosan | Nocardiopsis alba |
| 9 | - |
immobilized enzyme on silica gel 100-200, polyacrylamide, and nitrocellulose membrane | Nocardiopsis alba |
| 10 | - |
free enzyme | Nocardiopsis alba |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme is a highly thermostable alkaline serine protease | Nocardiopsis alba |
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