Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.65 extracted from

  • Ong, P.S.; Gaucher, G.M.
    Production, purification and characterization of thermomycolase, the extracellular serine protease of the thermophilic fungus Malbranchea pulchella var. sulfurea (1976), Can. J. Microbiol., 22, 165-176.
    View publication on PubMed
Search for more literature for 3.4.21.65

General Stability

General Stability Organism
Macromolecules and Ca2+ provide protection against significant autolysis Malbranchea pulchella

Inhibitors

Inhibitors Comment Organism Structure
Serine protease inhibitors
-
 Malbranchea pulchella

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Malbranchea pulchella
-
-

Organism

Organism UniProt Comment Textmining
Malbranchea pulchella
-
 var. sulfurea
-

Purification (Commentary)

Purification (Comment) Organism
-
 Malbranchea pulchella

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Malbranchea pulchella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information not: N-acetyl-L-Tyr ethyl ester Malbranchea pulchella ?
-
 ?
additional information small synthetic ester substrates are preferentially hydrolyzed at bonds situated on the carboxyl side of aromatic or apolar amino acid residues which are not beta-branched, positively charged or of the D-configuration Malbranchea pulchella ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 maximal thermostability 10 mM Ca2+ Malbranchea pulchella
73
-
 110 min with 10 mM Ca2+ Malbranchea pulchella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 6.0 Malbranchea pulchella
8.5
-
 bovine serum albumin Malbranchea pulchella

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10.5 30°C, 2 h, stable Malbranchea pulchella
6 9.6 30°C, 20 h, stable Malbranchea pulchella
6.5 9.5 30°C, 20 h, stable Malbranchea pulchella
8.5
-
 optimum pH for stability Malbranchea pulchella