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Literature summary for 3.4.21.64 extracted from

  • Jikuzono, T.; Horikawa, A.; Ishikawa, T.; Hirokawa, M.; Sugitani, I.; Inui, T.; Ishibashi, O.
    Proteinase K treatment improves RNA recovery from thyroid cells fixed with liquid-based cytology solution (2018), BMC Res. Notes, 11, 822 .
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
analysis improvement of RNA extraction from papillary cancer-derived K1 cells and thyroid fine-needle aspiration biopsy (FNAB) specimens suspended in liquid-based cytology (LBC) solutions during fine-needle aspiration biopsy diagnostics of thyroid diseases. Commercial proteinase K treatment is essential for efficient RNA extraction from the fixed cells. Proteinase K treatment facilitates RNA recovery from rigid cells after dehydration. RNA molecules are largely released from the fixed cells even after 1-h treatment. U6 small nuclear RNA was detected in these RNA samples by reverse transcription-PCR. Method overview Parengyodontium album

Organism

Organism UniProt Comment Textmining
Parengyodontium album P06873 i.e. Tritirachium album or Engyodontium album
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Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
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 Parengyodontium album
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Synonyms

Synonyms Comment Organism
PROK
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 Parengyodontium album
Proteinase K
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 Parengyodontium album

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Parengyodontium album

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Parengyodontium album