Literature summary for 3.4.21.64 extracted from

Imamura, M.; Kato, N.; Okada, H.; Yoshioka, M.; Iwamaru, Y.; Shimizu, Y.; Mohri, S.; Yokoyama, T.; Murayama, Y.
Insect cell-derived cofactors become fully functional after proteinase K and heat treatment for high-fidelity amplification of glycosylphosphatidylinositol-anchored recombinant scrapie and BSE prion proteins (2013), PLoS ONE, 8, e82538.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of tagged cellular Bac-prion protein fusion in Spodoptera frugiperda SF21 insect cells via baculovirus transfection system	Parengyodontium album

Organism

Organism	UniProt	Comment	Textmining
Parengyodontium album	P06873	-	-

Purification (Commentary)

Purification (Comment)	Organism
tagged cellular Bac-prion protein fusion partially 37fold from SF21 insect cells using immobilized metal ion affinity chromatography	Parengyodontium album

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
normal cellular prion protein + H2O	in mouse brain	Parengyodontium album	pathogenic cellular prion protein + ?	-	?

Synonyms

Synonyms	Comment	Organism
Proteinase K	-	Parengyodontium album

General Information

General Information	Comment	Organism
metabolism	the central event in prion infection is the conformational conversion of host-encoded cellular prion protein into the pathogenic isoform. For the in vitro replication of pathogenic prion protein by protein-misfolding cyclic amplification, cofactors are required that are not produced by lower organism, e.g. insects, but by mammalia, e.g. C57BL/6J mice. Brain-derived factors are also necessary for the in vitro replication of glycosylphosphatidylinositol-anchored baculovirus-derived recombinant prion protein. Cofactor activity of insect cell lysates becomes functional after proteinase K digestion and heat treatment, following protease digestion and heat treatment, insect cell lysates had the functional cofactor activity required for Bac-prion protein replication by protein-misfolding cyclic amplification. Not only RNA, but also DNA, are the key components of protein-misfolding cyclic amplification, although other cellular factors were necessary for the expression of the cofactor activity of nucleic acids in insect cells	Parengyodontium album
additional information	proteinase K-resistant Bac-prion protein is generated by protein-misfolding cyclic amplification using insect cells expressing the protein and prionprotein null mouse brain homogenate	Parengyodontium album

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Release 2023.1 (January 2023)