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Literature summary for 3.4.21.63 extracted from
- Purification and characterization of a novel extracellular thermostable alkaline protease from Streptomyces sp. M30 (2015), J. Microbiol. Biotechnol., 25, 1944-1953 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dimethyl sulfoxide | 12% activation at 5% | Streptomyces sp. M30 |  |
| dimethylformamide | 15% activation at 5% | Streptomyces sp. M30 | |
| Tween 80 | 21% activation at 1% | Streptomyces sp. M30 | |
| Urea | 23% activation at 1 M | Streptomyces sp. M30 | |
Application
| Application | Comment | Organism |
|---|---|---|
| industry | alkaline proteases are a major group of industrial enzymes that hydrolyze proteins into short peptides and amino acids, and catalyze peptide synthesis | Streptomyces sp. M30 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene sapHM, DNA and amino acid sequence determination and analysis, phylogenetic tree | Streptomyces sp. M30 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetone | 13% inhibition at 10% | Streptomyces sp. M30 | |
| acetonitrile | 48% inhibition at 10% | Streptomyces sp. M30 | |
| DTT | - |
Streptomyces sp. M30 | |
| EDTA | - |
Streptomyces sp. M30 | |
| EGTA | - |
Streptomyces sp. M30 | |
| ethanol | 39.5% inhibition at 10% | Streptomyces sp. M30 | |
| Isopropanol | 68% inhibition at 10% | Streptomyces sp. M30 | |
| additional information | no or poor inhibition by Ba2+, Cr3+, K+, Fe3+, Ca2+, Zn2+, and Mg2+, all at 1 mM. No inhibition by methanol at 10% | Streptomyces sp. M30 | |
| PMSF | complete inhibition | Streptomyces sp. M30 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km and Vmax values are estimated to be 35.7 mg/ml, and 5 × 104 U/mg for casein, Michaelis-Menten kinetics are calculated using Lineweaver-Burk double-reciprocal plots | Streptomyces sp. M30 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptomyces sp. M30 | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | activates 47% at 1 mM | Streptomyces sp. M30 | |
| Mn2+ | activates 13% at 1 mM | Streptomyces sp. M30 | |
| Ni2+ | activates 12% at 1 mM | Streptomyces sp. M30 | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| additional information | stable in the presence of methanol and acetone | Streptomyces sp. M30 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces sp. M30 | A0A0C4XY83 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 7fold from strain M30 by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography | Streptomyces sp. M30 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 29.07 | - |
purified extracellular enzyme, pH 9.0, 75°C | Streptomyces sp. M30 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Bovine serum albumin + H2O | - |
Streptomyces sp. M30 | ? | - |
? | |
| bovine serum fibrin + H2O | - |
Streptomyces sp. M30 | ? | - |
? | |
| casein + H2O | - |
Streptomyces sp. M30 | ? | - |
? | |
| additional information | activity with gelatin | Streptomyces sp. M30 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37102, mass spectrometry | Streptomyces sp. M30 |
| More | enzyme peptide mass fingerprint analysis | Streptomyces sp. M30 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alkaline protease | - |
Streptomyces sp. M30 |
| SapHM | - |
Streptomyces sp. M30 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Streptomyces sp. M30 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 90 | activity range, profile overview | Streptomyces sp. M30 |
| 70 | 85 | over 50% activity within this range, profile overview | Streptomyces sp. M30 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
40 | purified recombinant enzyme, 2.5 h, completely stable at | Streptomyces sp. M30 |
| 50 | - |
purified recombinant enzyme, 30 min, 55% activity remaining, inactivation after 2.5 h | Streptomyces sp. M30 |
| 60 | - |
purified recombinant enzyme, 30 min, 40% activity remaining, inactivation after 2 h | Streptomyces sp. M30 |
| 70 | - |
purified recombinant enzyme, 30 min, inactivation | Streptomyces sp. M30 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Streptomyces sp. M30 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10.5 | over 50% of maximal activity within this range, profile overview | Streptomyces sp. M30 |
| 7 | 9 | over 90% of maximal activity within this range, profile overview | Streptomyces sp. M30 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | purified recombinant enzyme, over 90% activity remaining | Streptomyces sp. M30 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme sequence cotains conserved Asp156, His187, and Ser339 residues of alkaline protease. The enzyme is a serine protease | Streptomyces sp. M30 |
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