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Literature summary for 3.4.21.63 extracted from
- Response surface methodology for production, characterization and application of solvent, salt and alkali-tolerant alkaline protease from isolated fungal strain Aspergillus niger WA 2017 (2018), Int. J. Biol. Macromol., 115, 447-458 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | activates 20% at 0.5% | Aspergillus niger |  |
| Tween 80 | slight activation at 0.5% | Aspergillus niger | |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the produced enzyme is able to recover silver from used X-ray film under optimized condition, overview | Aspergillus niger |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| butanol | 50% inhibition after 2 h | Aspergillus niger | |
| EDTA | - |
Aspergillus niger | |
| H2O2 | - |
Aspergillus niger | |
| iodoacetic acid | strong inhibition at 5 mM | Aspergillus niger | |
| additional information | the enzyme is completely stable in presence of laundry detergents, overview | Aspergillus niger | |
| PMSF | strong inhibition at 5 mM | Aspergillus niger | |
| SDS | strong inhibition at 0.5% | Aspergillus niger | |
| Urea | slight inhibition at 5 mM | Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | activates at up to 12% w/v, the enzyme is stable up to 20% w/v, thermal stability of the enzyme is substantially improved by NaCl | Aspergillus niger | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Butanol | 50% inhibition after 2 h | Aspergillus niger |
| Ethanol | the enzyme shows excellent stability and compatibility in presence of organic solvents and detergents retaining 115.3% of its activity in presence of ethanol at 40°C for 1 h | Aspergillus niger |
| additional information | the enzyme is highly stable in different organic solvents at 40°C for 1-2 h, e.g. in ethanol, methanol, butanol, acetone, benzene, ethyl acetate, and chloroform, all at the concentration of 25% v/v | Aspergillus niger |
| additional information | the enzyme shows excellent stability and compatibility in presence of commercial detergent Tide retaining 114.5% of its activity in presence of 1% (w/v) at 40°C for 1 h | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger WA 2017 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | the enzyme production is increased by 3.6fold with statistically optimized medium when compared to the basal medium, optimization, overview | Aspergillus niger | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Aspergillus niger | ? | - |
? | |
| casein + H2O | - |
Aspergillus niger WA 2017 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alkaline protease | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the thermal stability of the enzyme is substantially improved by NaCl | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | stable at | Aspergillus niger |
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