Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by H2O2 and NaClO | Aspergillus terreus | |
SDS | - |
Aspergillus terreus | |
Triton X-100 | - |
Aspergillus terreus | |
Tween 80 | - |
Aspergillus terreus |
Application | Comment | Organism |
---|---|---|
detergent | alkaline proteases are primarily used in the detergent industry. The addition of the protease to the detergent Super wheel improved its blood stain removal | Aspergillus terreus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | 9.9% inhibition at 5 mM | Aspergillus terreus | |
EDTA | 12.7% inhibition at 5 mM | Aspergillus terreus | |
Fe3+ | 18.9% inhibition at 5 mM | Aspergillus terreus | |
Hg2+ | 25.2% inhibition at 5 mM | Aspergillus terreus | |
iodoacetamide | 5.2% inhibition at 5 mM | Aspergillus terreus | |
Mn2+ | 14.1% inhibition at 5 mM | Aspergillus terreus | |
additional information | no effect by H2O2 and NaClO | Aspergillus terreus | |
N-bromosuccinimide | 50.3% inhibition at 5 mM | Aspergillus terreus | |
N-diazoacetylnorleucine methyl ester | 8.3% inhibition at 5 mM | Aspergillus terreus | |
N-ethylmaleimide | 11.2% inhibition at 5 mM | Aspergillus terreus | |
Na+ | 7.5% inhibition at 5 mM | Aspergillus terreus | |
PMSF | 83.7% inhibition at 5 mM | Aspergillus terreus | |
Sodium azide | 22.0% inhibition at 5 mM | Aspergillus terreus | |
tosyl-L-lysylchloromethyl ketone | 6.7% inhibition at 5 mM | Aspergillus terreus | |
Urea | 4.5% inhibition at 5 mM | Aspergillus terreus | |
Zn2+ | 10.9% inhibition at 5 mM | Aspergillus terreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for casein is 5.4 mg/ml | Aspergillus terreus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates 82.6% at 10 mM | Aspergillus terreus | |
Co2+ | activates 21.1% at 10 mM | Aspergillus terreus | |
Mg2+ | activates 52.2% at 10 mM | Aspergillus terreus | |
additional information | no or poor effects by 10 mM of K+, Ba2+, Cu2+, and Fe2+ | Aspergillus terreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
about, native PAGE | Aspergillus terreus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 28.6fold by acetone precipitation, freezing of the precipitate for 3 h at -20°C, dissolvation, and lyophilization, followed by concanavalin A lectin affinity chromatography | Aspergillus terreus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
148.9 | - |
purified enzyme, pH 11.0, 40°C | Aspergillus terreus |
Storage Stability | Organism |
---|---|
the enzyme exhibits high storage stability at 4°C, 28°C, and -20°C | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | - |
Aspergillus terreus | ? | - |
? | |
Bovine serum albumin + H2O | - |
Aspergillus terreus gr. | ? | - |
? | |
casein + H2O | best substrate | Aspergillus terreus | ? | - |
? | |
casein + H2O | best substrate | Aspergillus terreus gr. | ? | - |
? | |
Gelatin + H2O | the active enzyme degrades the copolymerized gelatin with high activity | Aspergillus terreus | ? | - |
? | |
Gelatin + H2O | the active enzyme degrades the copolymerized gelatin with high activity | Aspergillus terreus gr. | ? | - |
? | |
hen egg albumin + H2O | - |
Aspergillus terreus | ? | - |
? | |
hen egg albumin + H2O | - |
Aspergillus terreus gr. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 16240, SDS-PAGE and LC-MS | Aspergillus terreus |
Synonyms | Comment | Organism |
---|---|---|
Alkaline protease | - |
Aspergillus terreus |
Aspergillus terreus protease | - |
Aspergillus terreus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Aspergillus terreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | purified enzyme, 24 h, completely stable at | Aspergillus terreus |
60 | 90 | purified secreted enzyme, 1 h, completely stable at | Aspergillus terreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Aspergillus terreus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
11 | 12 | purified enzyme, 28°C, 24 h, completely stable at | Aspergillus terreus |