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Literature summary for 3.4.21.63 extracted from

  • Castro-Ochoa, D.; Pena-Montes, C.; Farres, A.
    Evaluation of strategies to improve the production of alkaline protease PrtA from Aspergillus nidulans (2013), Appl. Biochem. Biotechnol., 169, 1672-1682.
    View publication on PubMed
Search for more literature for 3.4.21.63

Cloned(Commentary)

Cloned (Comment) Organism
gene prtA, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X-33 as inactive enzyme, the presence of this enzyme was toxic for the host and resulted in cell lysis, modification of the culture medium constituents improve the expression by 6.4fold Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, SDS-PAGE Aspergillus nidulans

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
 gene prtA
-
Aspergillus nidulans PW1
-
 gene prtA
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium no proteolytic activity in minimal medium with 1% glucose or with skim milk as nitrogen source, low levels of proteolytic activity in the absence of an additionel nitrogen source, highest enzyme activity and production in medium with 0.5% beef extract, culture conditions, overview Aspergillus nidulans
-

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Aspergillus nidulans

Synonyms

Synonyms Comment Organism
Alkaline protease
-
 Aspergillus nidulans
PrtA
-
 Aspergillus nidulans
PrtA protease
-
 Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Aspergillus nidulans

Expression

Organism Comment Expression
Aspergillus nidulans the enzyme is not induced by lipids additional information