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Literature summary for 3.4.21.62 extracted from
- Molecular cloning and characterization of a subtilisin-like protease from Arabidopsis thaliana (2015), Genet. Mol. Res., 14, 16535-16545 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene SBT1.9 or At5g67090, recombinant expression of soluble mature enzyme AtSBT1.9 fused to maltose-binding protein lacking the signal peptide in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | the enzyme contains a signal peptide | Arabidopsis thaliana | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9FHA4 | - |
- |
| Arabidopsis thaliana Col-0 | Q9FHA4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant mature enzyme AtSBT1.9 fused to maltose-binding protein from Escherichia coli BL21(DE3) by amylose affinity chromatography | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9.23 | - |
purified recombinant MBP-tagged mature enzyme, pH 7.0, 25°C | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Arabidopsis thaliana | ? | - |
? |  |
| azocasein + H2O | - |
Arabidopsis thaliana Col-0 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 117500, recombinant soluble mature enzyme AtSBT1.9 fused to maltose-binding protein, SDS-PAGE, x * 77500, about, mature untagged enzyme, sequence calculation | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtSBT1.9 | - |
Arabidopsis thaliana |
| subtilase | - |
Arabidopsis thaliana |
| subtilase subfamily 1 member 9 | - |
Arabidopsis thaliana |
| subtilisin-like serine protease | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
recombinant enzyme | Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | 70 | recombinant enzyme, over 50% of maximal activity within the range of 18-50°C, 40% of maximal activity at 60°C, 10% at 70°C | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant enzyme | Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | recombinant enzyme, over 40% of maximal activity within this range | Arabidopsis thaliana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the Arabidopsis thaliana genome encodes 56 subtilisin-like serine proteases (subtilases), enzyme AtSBT1.9 is a member of the subtilase subfamily 1 | Arabidopsis thaliana |
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