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Literature summary for 3.4.21.62 extracted from

  • Uehara, R.; Takeuchi, Y.; Tanaka, S.; Takano, K.; Koga, Y.; Kanaya, S.
    Requirement of Ca(2+) ions for the hyperthermostability of Tk-subtilisin from Thermococcus kodakarensis (2012), Biochemistry, 51, 5369-5378.
    View publication on PubMed
Search for more literature for 3.4.21.62

Protein Variants

Protein Variants Comment Organism
additional information construction of pro-enzyme derivatives lacking the Ca1 ion (Pro-TKS/DELTACa1), Ca6 ion (Pro-TKS/DELTACa6), and Ca7 ion (Pro-TKS/DELTACa7), and their active site mutants, Pro-S324A/DELTACa1, Pro-S324A/DELTACa6, and Pro-S324A/DELTACa7, respectively. Mutants Pro-TKS/DELTACa6 and Pro-TKS/DELTACa7 fully mature into their active forms upon incubation at 80°C for 30 min like the wild-type enzyme. In contrast, mutant Pro-TKS/DELTACa1 matures poorly at 80°C because of the instability of its mature domain. Refolding rates of all Pro-S324A derivatives are comparable to that of Pro-S324A (active site mutant of wild-type pro-enzyme), indicating that these Ca(2+) ions are not needed for folding of Tk-subtilisin Thermococcus kodakarensis
S324A site-directed mutagenesis, active site mutant Thermococcus kodakarensis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme contains 7 Ca2+ ions, 4 of which are responsible for folding requirement of Ca2+ ions for the hyperthermostability of Tk-subtilisin, the Ca1, Ca6, and Ca7 ions, especially the Ca1 ion, contribute to the hyperthermostabilization of Tk-subtilisin Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JIZ5
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Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme matures from the inactive precursor, Pro-Tk-subtilisin (Pro-TKS), upon autoprocessing and degradation of the propeptide (Tkpro) Thermococcus kodakarensis

Synonyms

Synonyms Comment Organism
subtilisin-like serine protease
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 Thermococcus kodakarensis
Tk-subtilisin
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 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
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 assay at Thermococcus kodakarensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
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 requirement of Ca2+ ions for the hyperthermostability of Tk-subtilisin.The Ca1, Ca6, and Ca7 ions, especially the Ca1 ion, contribute to the hyperthermostabilization of Tk-subtilisin Thermococcus kodakarensis