Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli | Streptococcus suis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminoethyl)-benzenesulfonyl fluoride | - |
Streptococcus suis | |
N-tosyl-L-phenylalanyl chloromethyl ketone | - |
Streptococcus suis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
SDS-PAGE | Streptococcus suis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus suis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ion-exchange chromatography, ultrafiltration and gel filtration | Streptococcus suis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Aalpha chain of fibrinogen + H2O | - |
Streptococcus suis | ? | - |
? | |
Gelatin + H2O | - |
Streptococcus suis | ? | - |
? | |
succinyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O | - |
Streptococcus suis | succinyl-Ala-Ala-Pro-Phe + p-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Sspa | - |
Streptococcus suis |
SSU0757 | - |
Streptococcus suis |
subtilisin-like protease | - |
Streptococcus suis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptococcus suis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | - |
Streptococcus suis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
relatively unstable when incubated at 37°C for 18 h (80% reduction) | Streptococcus suis |
45 | - |
heating the recombinant protease at 45°C results in a 20% reduction of activity | Streptococcus suis |
65 | - |
heating the recombinant protease at 65°C results in a 65% reduction of activity | Streptococcus suis |
100 | - |
heating the recombinant protease at 100°C results in a total loss of activity | Streptococcus suis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptococcus suis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | protease activity decreases significantly below pH 6 and above pH 9 | Streptococcus suis |