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Literature summary for 3.4.21.62 extracted from
- Statistical optimization of production, purification and industrial application of a laundry detergent and organic solvent-stable subtilisin-like serine protease (Alzwiprase) from Bacillus subtilis DM-04 (2010), Biochem. Eng. J., 48, 173-180.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-bromophenacyl bromide | - |
Bacillus subtilis |  |
| iodoacetamide | - |
Bacillus subtilis | |
| PMSF | - |
Bacillus subtilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.059 | - |
casein | pH 10, 45°C, Vmax: 0.336 mg/min | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 100% inhibition | Bacillus subtilis | |
| Mg2+ | 100% inhibition | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16900 | - |
SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified to 23.5fold by a combination of cation and anion exchange chromatography, ethanol precipitation followed by reverse-phase HPLC | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Bovine serum albumin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| casein + H2O | - |
Bacillus subtilis | ? | - |
? | |
| chicken-feather keratin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| Gelatin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| Hemoglobin + H2O | - |
Bacillus subtilis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | SDS-PAGE, 1 * 16900 Da | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Alzwiprase | - |
Bacillus subtilis |
| subtilisin-like serine protease | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Bacillus subtilis |
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Release 2023.1 (January 2023)
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