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Literature summary for 3.4.21.62 extracted from

  • Anikina, O.M.; Semashko, T.A.; Oksenoit, E.S.; Lysogorskaya, E.N.; Filippova, I.Y.
    Native subtilisin Karlsberg and modified subtilisin 72 as effective catalysts of peptide bond formation in organic media (2006), Russ. J. Bioorg. Chem., 32, 116-121.
No PubMed abstract available
Search for more literature for 3.4.21.62

Application

Application Comment Organism
additional information the subtilisin Karlsberg complex with sodium dodecyl sulfate in anhydrous organic solvents is an effective catalyst of peptide synthesis with multifunctional amino acids in positions P 1 and (Glu, Arg, and Asp) containing unprotected side ionogenic groups Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMSO Bacillus subtilis
0.14
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMSO Bacillus subtilis
0.18
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMF Bacillus subtilis
0.19
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMF Bacillus subtilis
0.25
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMF Bacillus licheniformis
0.25
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMSO Bacillus licheniformis
4
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMF Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Bacillus subtilis
-
-
-
Bacillus subtilis 72
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Glp-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus subtilis ?
-
 ?
Glp-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus licheniformis ?
-
 ?
Glp-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus subtilis 72 ?
-
 ?
Z-Ala-Ala-Leu-OCH3 + H2O
-
 Bacillus licheniformis ?
-
 ?
Z-Ala-Ala-Leu-OMe + Phe-p-nitroanilide
-
 Bacillus licheniformis Z-Ala-Ala-Leu-Phe-p-nitroanilide + methanol
-
 ?
Z-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus subtilis ?
-
 ?
Z-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus licheniformis ?
-
 ?
Z-Ala-Ala-Leu-p-nitroanilide + H2O
-
 Bacillus subtilis 72 ?
-
 ?
Z-Thr-Ala-Thr-OCH3 + Asp-p-nitroanilide
-
 Bacillus licheniformis Z-Thr-Ala-Thr-Asp-p-nitroanilide + methanol
-
 ?

Synonyms

Synonyms Comment Organism
subtilisin 72
-
 Bacillus subtilis
subtilisin Karlsberg
-
 Bacillus licheniformis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
64.3
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMF Bacillus subtilis
69
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMF Bacillus licheniformis
70.9
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMF Bacillus subtilis
77
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMSO Bacillus licheniformis
80
-
 Glp-Ala-Ala-Leu-p-nitroanilide in 2% DMSO Bacillus subtilis
100
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMSO Bacillus subtilis
104
-
 Z-Ala-Ala-Leu-p-nitroanilide in 20% DMF Bacillus licheniformis