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Literature summary for 3.4.21.62 extracted from

  • Choi, N.S.; Chang, K.T.; Jae Maeng, P.; Kim, S.H.
    Cloning, expression, and fibrin (ogen)olytic properties of a subtilisin DJ-4 gene from Bacillus sp. DJ-4 (2004), FEMS Microbiol. Lett., 236, 325-331.
    View publication on PubMed
Search for more literature for 3.4.21.62

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length enzyme and mature form, expression of mature form does not give enzymatic activity Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, mature enzyme Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) Q6IT79 proenzyme
-
Bacillus sp. (in: Bacteria) DJ-4 Q6IT79 proenzyme
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fibrinogen + H2O rapid hydrolysis of Aalpha-, Bbeta- and gamma-chains. At very low concentration, no cleavage of gamma-chain Bacillus sp. (in: Bacteria) ?
-
 ?
Fibrinogen + H2O rapid hydrolysis of Aalpha-, Bbeta- and gamma-chains. At very low concentration, no cleavage of gamma-chain Bacillus sp. (in: Bacteria) DJ-4 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, mature enzyme Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 recombinant enzyme, stable for 40 min, native enzyme, 30% loss of activity after 40 min Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 broad Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 recombinant enzyme, about 85% residual activity after 48 h at 4°C, native enzyme, 60% residual activity Bacillus sp. (in: Bacteria)