Application | Comment | Organism |
---|---|---|
analysis | popular system for protein engineering studies | Bacillus amyloliquefaciens |
synthesis | subtilisin BPN' is an important industrial enzyme | Bacillus amyloliquefaciens |
Cloned (Comment) | Organism |
---|---|
subtilisin BPN' cloned, sequenced and expressed at high levels from its natural promoter sequences in Bacillus subtilis | Bacillus amyloliquefaciens |
Protein Variants | Comment | Organism |
---|---|---|
G166S | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
G169A | stable only in presence of excess Ca2+ | Bacillus amyloliquefaciens |
L126I | site-directed mutagenesis, BPN', stable only in presence of excess Ca2+ | Bacillus amyloliquefaciens |
M50F | site-directed mutagenesis, BPN',stabilized in EDTA, but destabilized slightly in CaCl2 | Bacillus amyloliquefaciens |
N218S | site-directed mutagenesis, BPN', stabilized significantly, either with or without Ca2+ | Bacillus amyloliquefaciens |
Q206Cox | site-directed mutagenesis, BPN' | Bacillus amyloliquefaciens |
Q271E | site-directed mutagenesis, BPN', stabilized significantly, either with or without Ca2+ | Bacillus amyloliquefaciens |
S188P | site-directed mutagenesis, BPN', stable only in presence of excess Ca2+ | Bacillus amyloliquefaciens |
T254A | site-directed mutagenesis, BPN', stable only in presence of excess Ca2+ | Bacillus amyloliquefaciens |
Y217K | site-directed mutagenesis, BPN', stabilized significantly, either with or without Ca2+ | Bacillus amyloliquefaciens |
General Stability | Organism |
---|---|
stability is a property of subtilisin which has proven particularly amenable to enhancement via random mutagenesis | Bacillus amyloliquefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizing mutations are usually calcium-dependent in their stabilizing effect | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
subtilisin BPN' and recombinant variants | Bacillus amyloliquefaciens |
Synonyms | Comment | Organism |
---|---|---|
BPN' | - |
Bacillus amyloliquefaciens |