Literature summary for 3.4.21.61 extracted from

Rabah, N.; Gauthier, D.; Dikeakos, J.D.; Reudelhuber, T.L.; Lazure, C.
The C-terminal region of the proprotein convertase 1/3 (PC1/3) exerts a bimodal regulation of the enzyme activity in vitro (2007), FEBS J., 274, 3482-3491.

Search for more literature for 3.4.21.61

Activating Compound

Activating Compound	Comment	Organism	Structure
CT-peptide	increases PC1/3 activity at low concentrations (nanomol). PC1/3, through its various domains, is capable of controlling its enzymatic activity in all regions of the cell that it encounters. Activation of PC1/3 by 5 nanomol CT-peptide is the same in the presence or absence of propeptide	Mus musculus

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant PC1/3 produced using the baculovirus expression system in Sf-9 insect cells or through intracoelemic injection in insect larvae	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
CT-peptide	decreases PC1/3 activity at high concentrations (micromol)	Mus musculus
propeptide	addition of purified recombinant propeptide (20 nanomol) to PC1/3 enzymatic reaction leads to a 50% reduction in enzymatic activity. In the presence of 5 nanomol CT-peptide and 20 nanomol propeptide, inhibition is reduced to 35%	Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	-	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17640	-	mass spectrometry analysis	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	CT-peptide is not cleaved by enzymatically active PC1/3	Mus musculus	?	-	?
pGlu-Arg-Thr-Lys-Arg-4-methylcoumaryl-7-amide + H2O	-	Mus musculus	7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
PC1/3	-	Mus musculus
proprotein convertase 1/3	-	Mus musculus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.002	-	CT-peptide	-	Mus musculus

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Release 2023.1 (January 2023)