Cloned (Comment) | Organism |
---|---|
cloned KEX2 gene introduced into the kex2 mutant cells and the KEX2 gene product expressed in these cells | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diisopropyl fluorophosphate | - |
Saccharomyces cerevisiae | |
HgCl2 | - |
Saccharomyces cerevisiae | |
leupeptin | - |
Saccharomyces cerevisiae | |
p-chloromercuribenzoate | - |
Saccharomyces cerevisiae | |
pepstatin A | - |
Saccharomyces cerevisiae | |
phenylmethylsulfonyl fluoride | - |
Saccharomyces cerevisiae | |
ZnCl2 | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
cloned KEX2 gene introduced into the kex2 mutant cells and the KEX2 gene product expressed in these cells | - |
Purification (Comment) | Organism |
---|---|
partial | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-tert-butyloxycarbonyl-Gly-Lys-Arg 4-methylcoumarin 7-amide + H2O | 59.2% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide | Saccharomyces cerevisiae | N-tert-butyloxycarbonyl-Gly-Lys-Arg + 7-amino-4-methylcoumarin | - |
? | |
Protein + H2O | specificity towards the carbonyl side of Lys-Arg, Arg-Arg and Pro-Arg sequences | Saccharomyces cerevisiae | ? | - |
? | |
tert-Butyloxycarbonyl-Ala-Pro-Arg 4-methylcoumarin 7-amide + H2O | 17% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide | Saccharomyces cerevisiae | ? | - |
? | |
tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide + H2O | - |
Saccharomyces cerevisiae | tert-butyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
tert-Butyloxycarbonyl-Leu-Arg-Arg 4-methylcoumarin 7-amide + H2O | 116% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide | Saccharomyces cerevisiae | ? | - |
? | |
tert-Butyloxycarbonyl-Leu-Lys-Arg 4-methylcoumarin 7-amide + H2O | 92.8% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide | Saccharomyces cerevisiae | ? | - |
? | |
tert-Butyloxycarbonyl-Val-Pro-Arg 4-methylcoumarin 7-amide + H2O | 38% of the activity with tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide | Saccharomyces cerevisiae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
tert-butyloxycarbonyl-Gln-Arg-Arg 4-methylcoumarin 7-amide | Saccharomyces cerevisiae |