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Literature summary for 3.4.21.6 extracted from
- Recombinant snake venom prothrombin activators (2013), Bioengineered, 4, 153-157.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Factor Va | dependent on | Tropidechis carinatus | |
| Phospholipids | dependent on | Tropidechis carinatus |  |
| Phospholipids | dependent on | Oxyuranus scutellatus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparison, transient recombinant expression in COS-7 cells, the enzyme is secreted | Oxyuranus scutellatus |
| transient recombinant expression in COS-7 cells, the enzyme is secreted | Tropidechis carinatus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Tropidechis carinatus | - |
- |
| extracellular | - |
Oxyuranus scutellatus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on | Tropidechis carinatus | |
| Ca2+ | dependent on | Oxyuranus scutellatus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| prothrombin + H2O | Tropidechis carinatus | gamma-carboxylation of prothrombin is required for cleavage | ? | - |
? | |
| prothrombin + H2O | Oxyuranus scutellatus | gamma-carboxylation of prothrombin is required for cleavage | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oxyuranus scutellatus | Q58L91 | - |
- |
| Tropidechis carinatus | P81428 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Tropidechis carinatus | - |
| venom | - |
Oxyuranus scutellatus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | recombinant oscutarin cannot efficiently generate thrombin without additional protein cofactors | Oxyuranus scutellatus | ? | - |
? | |
| additional information | recombinant trocarin cannot efficiently generate thrombin without additional protein cofactors | Tropidechis carinatus | ? | - |
? | |
| prothrombin + H2O | gamma-carboxylation of prothrombin is required for cleavage | Tropidechis carinatus | ? | - |
? | |
| prothrombin + H2O | gamma-carboxylation of prothrombin is required for cleavage | Oxyuranus scutellatus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| oscutarin | - |
Oxyuranus scutellatus |
| prothrombin activator | - |
Tropidechis carinatus |
| prothrombin activator | - |
Oxyuranus scutellatus |
| trocarin | - |
Tropidechis carinatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Tropidechis carinatus |
| 37 | - |
assay at | Oxyuranus scutellatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Tropidechis carinatus |
| 7.4 | - |
assay at | Oxyuranus scutellatus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | recombinant oscutarin cannot efficiently generate thrombin without additional protein cofactors | Oxyuranus scutellatus | |
| additional information | recombinant trocarin cannot efficiently generate thrombin without additional protein cofactors | Tropidechis carinatus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme is similar to human coagulation factor X | Tropidechis carinatus |
| additional information | the enzyme is similar to human coagulation factor X | Oxyuranus scutellatus |
| physiological function | apart from the natural activation pathway, human prothrombin can also be activated by prothrombin activators in snake venoms. Oscutarin depends on calcium and phospholipids but not on factor Va for activity and generation of thrombin | Oxyuranus scutellatus |
| physiological function | apart from the natural activation pathway, human prothrombin can also be activated by prothrombin activators in snake venoms. Trocarin depends on calcium and phospholipids as well as factor Va for activity and generation of thrombin | Tropidechis carinatus |
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Release 2023.1 (January 2023)
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