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Literature summary for 3.4.21.6 extracted from
- Analysis of the kinetics of prothrombin activation and evidence that two equilibrating forms of prothrombinase are involved in the process (2003), J. Biol. Chem., 278, 6755-6764.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Homo sapiens | |
| 0.00036 | - |
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 0.00039 | - |
recombinant prothrombin R155A/R284A/R320A mutant rP2-II | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 0.00041 | - |
prothrombin | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 0.00045 | - |
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 0.0005 | - |
recombinant prothrombin R155A/R284A/R320A mutant rP2-II | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 0.00058 | - |
fragment 1.2:prethrombin-2 | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 0.00065 | - |
meizothrombin | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 0.00068 | - |
prothrombin | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 0.00078 | - |
fragment 1.2:prethrombin-2 | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 0.00095 | - |
meizothrombin | pH 7.4, 22°C, in absence of factor Va | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| selective cleavage of Arg-/-Thr and then Arg-/-Ile bonds in prothrombin to form thrombin | scutelarin has similar specificity | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fragment 1.2:prethrombin-2 + H2O | intermediate of prothrombin processing to thrombin, cleavage site is Arg320 | Homo sapiens | thrombin | - |
? | |
| meizothrombin + H2O | intermediate of prothrombin processing to thrombin, cleavage site is Arg271 | Homo sapiens | thrombin | - |
? | |
| prothrombin + H2O | wild-type II, cleavage sites are Arg271 and Arg320 | Homo sapiens | thrombin + ? | - |
? | |
| recombinant prothrombin R155A/R284A/R271A mutant rMZ-II + H2O | cleavage site is Arg320 | Homo sapiens | meizothrombin | reaction intermediate in the reaction with wild-type prothrombin | ? | |
| recombinant prothrombin R155A/R284A/R271A/S525C mutant rMZ-II-F + H2O | labeled with fluorescein at Cys525, cleavage site is Arg320 | Homo sapiens | meizothrombin | reaction intermediate in the reaction with wild-type prothrombin | ? | |
| recombinant prothrombin R155A/R284A/R320A mutant rP2-II + H2O | cleavage site is Arg271 | Homo sapiens | fragment 1.2:prethrombin-2 | reaction intermediate in the reaction with wild-type prothrombin | ? | |
| recombinant prothrombin S525C mutant + H2O | labeled with fluorescein at Cys525, cleavage sites are Arg271 and Arg320 | Homo sapiens | thrombin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the peptidase family S1, i.e. trypsin family | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at, room temperature | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
meizothrombin | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 0.0045 | - |
prothrombin | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 0.0057 | - |
fragment 1.2:prethrombin-2 | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 0.0068 | - |
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 0.324 | - |
recombinant prothrombin R155A/R284A/R320A mutant rP2-II | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 6.39 | - |
meizothrombin | pH 7.4, 22°C, in absence of factor Va | Homo sapiens | |
| 108 | - |
prothrombin | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 110 | - |
recombinant prothrombin R155A/R284A/R271A mutant rMZ-II | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 114 | - |
fragment 1.2:prethrombin-2 | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 114 | - |
recombinant prothrombin R155A/R284A/R320A mutant rP2-II | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens | |
| 150 | - |
meizothrombin | pH 7.4, 22°C, in presence of 20 mM factor Va | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Factor Va | required | Homo sapiens |
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